PMID: 8582319Jan 1, 1995Paper

Analysis of delay times of hemagglutinin-mediated fusion between influenza virus and cell membranes

European Biophysics Journal : EBJ
K LudwigA Herrmann

Abstract

We have studied the kinetics of low pH-induced fusion between influenza virus A/PR 8/34 and human erythrocyte membranes in suspension by using an assay based on fluorescence dequenching (FDQ) of the lipophilic dye octadecylrhodamine B chloride (R 18). As shown previously (Clague et al. 1991) the onset of FDQ is preceded by a characteristic lag time (tlag) following pH reduction. Whereas tlag represents only a subpopulation of fusing viruses with the shortest delay time we suggest here that a representative mean lag time mu lag of virus-cell fusion can be deduced from the R 18-assay. Kinetics of FDQ reflects the cumulative distribution function of lag times tau lag of single fusion events with the mean value mu lag. We show that tau lag obtained from the onset of FDQ does not always reflect the fusion behaviour of the whole population of fusing viruses. While both lag times, taulag and mu lag, exhibit a similar temperature dependence we found a significantly different dependence of both delay times on virus inactivation by low pH-pretreatment. We conclude that the mean lag time mu lag appears to be a more appropriate parameter describing the kinetics of virus-cell fusion. The analysis of delay times offers a new approach to test...Continue Reading

Citations

Aug 3, 2010·AAPS PharmSciTech·Yeu-Chun KimMark R Prausnitz
Oct 21, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yeu-Chun KimMark R Prausnitz
Aug 18, 2001·Biophysical Journal·S SchreiberH G Holzhütter
Jun 22, 2019·European Biophysics Journal : EBJ·Robert-William WelkePeter Müller

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