Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices

Journal of Visualized Experiments : JoVE
Pierfrancesco PagellaTim Mitsiadis

Abstract

Innervation plays a key role in the development, homeostasis and regeneration of organs and tissues. However, the mechanisms underlying these phenomena are not well understood yet. In particular, the role of innervation in tooth development and regeneration is neglected. Several in vivo studies have provided important information about the patterns of innervation of dental tissues during development and repair processes of various animal models. However, most of these approaches are not optimal to highlight the molecular basis of the interactions between nerve fibres and target organs and tissues. Co-cultures constitute a valuable method to investigate and manipulate the interactions between nerve fibres and teeth in a controlled and isolated environment. In the last decades, conventional co-cultures using the same culture medium have been performed for very short periods (e.g., two days) to investigate the attractive or repulsive effects of developing oral and dental tissues on sensory nerve fibres. However, extension of the culture period is required to investigate the effects of innervation on tooth morphogenesis and cytodifferentiation. Microfluidics systems allow co-cultures of neurons and different cell types in their app...Continue Reading

Citations

Sep 21, 2016·Stem Cells International·Shayee MiranPierfrancesco Pagella
Feb 26, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pierfrancesco PagellaThimios A Mitsiadis
Aug 24, 2018·Frontiers in Physiology·Giovanna OrsiniThimios A Mitsiadis

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