Analysis of Dictyostelium TACC reveals differential interactions with CP224 and unusual dynamics of Dictyostelium microtubules.

Cellular and Molecular Life Sciences : CMLS
Matthias SamereierRalph Gräf

Abstract

We have localized TACC to the microtubule-nucleating centrosomal corona and to microtubule plus ends. Using RNAi we proved that Dictyostelium TACC promotes microtubule growth during interphase and mitosis. For the first time we show in vivo that both TACC and XMAP215 family proteins can be differentially localized to microtubule plus ends during interphase and mitosis and that TACC is mainly required for recruitment of an XMAP215-family protein to interphase microtubule plus ends but not for recruitment to centrosomes and kinetochores. Moreover, we have now a marker to study dynamics and behavior of microtubule plus ends in living Dictyostelium cells. In a combination of live cell imaging of microtubule plus ends and fluorescence recovery after photobleaching (FRAP) experiments of GFP-α-tubulin cells we show that Dictyostelium microtubules are dynamic only in the cell periphery, while they remain stable at the centrosome, which also appears to harbor a dynamic pool of tubulin dimers.

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Citations

Nov 20, 2012·Cellular and Molecular Life Sciences : CMLS·Irina TikhonenkoMichael P Koonce
Jan 10, 2012·Cellular and Molecular Life Sciences : CMLS·Oliver KuhnertRalph Gräf
Jun 30, 2012·Cellular and Molecular Life Sciences : CMLS·Oliver KuhnertRalph Gräf
Mar 24, 2016·European Journal of Cell Biology·Sascha PutzlerRalph Gräf
Mar 13, 2014·Biological Reviews of the Cambridge Philosophical Society·Danton H O'Day, Aldona Budniak
May 9, 2019·Molecular Biology of the Cell·Jacob OdellMichael Koonce
Jul 8, 2020·Methods and Protocols·Michael KoonceRalph Gräf
Apr 11, 2020·Biomolecules·Michael P Koonce, Irina Tikhonenko

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