PMID: 20627053Jul 16, 2010Paper

Analysis of different proteins expression between papillary thyroid carcinoma and normal thyroid tissue

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery
Xue Bian, Ping-zhang Tang

Abstract

In order to understand the mechanisms of tumor development and identify potential new biomarkers of human papillary thyroid carcinoma (PTC), the different proteins expression were investigated and analyzed between PTC and normal thyroid tissue. By using immobilized pH gradient (IPG)-based two-dimensional gel electrophoresis (2-DE), the total proteins were separated and 2-DE maps were established for PTC and normal thyroid tissue, respectively. The differential protein spots were analyzed with Image Master 2D Elite5.0 software, then the peptide mass fingerprinting of different expressed proteins were identified by MALDI-TOF-MS/MS. Also, the functions of the differential expressed proteins were identified through Swiss-port database searching. The 2-DE maps of PTC and normal thyroid tissue was established. Sixty differential protein spots were found by image software and seventeen proteins were identified with MALDI-TOF-MS. The biological information of these proteins was identified through Swiss-port database. In comparison with normal thyroid tissue, a total of eleven proteins were up-regulated such as Cyclin-D2, Manganese SOD and Galectin-3; while six proteins were down-regulated including Peroxiredoxin-2, Hsp70 and Hsp27. Amo...Continue Reading

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