Analysis of Differential Expression of Synaptic Vesicle Protein 2A in the Adult Rat Brain

Neuroscience
Julieta G Mendoza-TorreblancaGisela Gómez-Lira

Abstract

Synaptic vesicle protein 2A (SV2A), which plays an important role in the pathophysiology of epilepsy, is a unique vesicular protein recognized as a pharmacological target of anticonvulsant drugs. Furthermore, SV2A is a potential synaptic density marker, as it is ubiquitously expressed throughout the brain in all nerve terminals independently of their neurotransmitter content. Due to the growing interest in this protein, we thoroughly analyzed SV2A levels, expression patterns and colocalization in both excitatory and inhibitory synapses among different brain structures in healthy rats. In addition, we discuss the main semiquantitative methodologies used to study SV2A because these techniques might represent powerful tools for evaluating synaptic changes associated with brain disorders. Our results showed that the SV2A expression levels differed among the analyzed structures, and a positive correlation between the SV2A mRNA copy number and protein level was observed by Western blot. In addition, immunohistochemistry demonstrated slight but consistent asymmetrical SV2A levels in different laminated structures, and SV2A expression was increased by up to 40% in some specific layers compared to that in others. Finally, triple immunof...Continue Reading

Related Concepts

Biological Markers
Immunofluorescence Assay
Brain Diseases
Immunohistochemistry
Anticonvulsants
SV2A protein, human
Epilepsy
Western Blotting
Thyroid Gland Follicular Cell
Brain

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