Analysis of Dishevelled signalling pathways during Xenopus development

Current Biology : CB
S Sokol

Abstract

Recent studies have demonstrated that the Wnt, Frizzled and Notch proteins are involved in a variety of developmental processes in fly, worm, frog and mouse embryos. The Dishevelled (Dsh) protein is required for Drosophila cells to respond to Wingless, Notch and Frizzled signals, but the molecular mechanisms of its action are not well understood. Using the ability of a mutant form of the Xenopus homologue of Dsh (Xdsh) to block Wnt and Dsh signalling in a model system, this work attempts to clarify the role of the endogenous Xdsh during the early stages of vertebrate development. A mutant Xdsh (Xdd1) with an internal deletion of the conserved PDZ/DHR domain was constructed. Overexpression of Xdd1 mRNA in ventral blastomeres of Xenopus embryos strongly inhibited induction of secondary axes by the wild-type Xdsh and Xwnt8 mRNAs, but did not affect the axis-inducing ability of beta-catenin mRNA. These observations suggest that Xdd1 acts as a dominant-negative mutant. Dorsal expression of Xdd1 caused severe posterior truncations in the injected embryos, whereas wild-type Xdsh suppressed this phenotype. Xdd1 blocked convergent extension movements in ectodermal explants stimulated with mesoderm-inducing factors and in dorsal marginal...Continue Reading

References

Jun 26, 1992·Cell·R Nusse, H E Varmus
Jul 12, 1991·Science·B BlumbergK W Cho
Jan 31, 1991·Biochemical and Biophysical Research Communications·K TashiroK Shiokawa
Jan 1, 1991·Methods in Cell Biology·R Keller
Jan 1, 1985·Differentiation; Research in Biological Diversity·J C Smith, F M Watt
Sep 25, 1984·Nucleic Acids Research·P A Krieg, D A Melton
Mar 1, 1995·Trends in Biochemical Sciences·C P Ponting, C Phillips
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·I DominguezS Y Sokol
May 1, 1995·Genes & Development·S YanagawaR Nusse
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·A Karnovsky, M W Klymkowsky
Dec 1, 1994·Developmental Biology·D HerzlingerA M Brown
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·R A LueA H Chishti
Aug 1, 1994·Current Opinion in Genetics & Development·B A Parr, A P McMahon
Feb 25, 1996·Developmental Biology·L K GontE M De Robertis

❮ Previous
Next ❯

Citations

Sep 2, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R T Moon, D Kimelman
Nov 18, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·T Bouwmeester, L Leyns
Dec 3, 1999·Biochemical and Biophysical Research Communications·M InobeS Takeda
Feb 19, 2000·Biochemical and Biophysical Research Communications·A Kikuchi
Sep 26, 2002·Developmental Biology·Alin Vonica, Barry M Gumbiner
Jun 14, 2005·Cytokine & Growth Factor Reviews·Motoko Yanagita
May 6, 2003·Developmental Biology·Alfredo Penzo-MendèzJean-François Riou
Jun 27, 2002·Biochimica Et Biophysica Acta·Yosef LandesmanDavid L Paul
Aug 15, 2002·Trends in Genetics : TIG·Dina C MyersLilianna Solnica-Krezel
Apr 1, 1997·Trends in Genetics : TIG·R T MoonM Torres
Jan 5, 2000·Cellular Signalling·A Kikuchi
Nov 18, 1998·Current Opinion in Cell Biology·A Ben-Ze'ev, B Geiger
Apr 21, 1999·Current Opinion in Cell Biology·Q Eastman, R Grosschedl
Jan 27, 2000·Cytokine & Growth Factor Reviews·A Kikuchi
Jun 14, 2002·Developmental Cell·John B WallingfordRichard M Harland
May 12, 2012·Cell Death and Differentiation·A MamidiS Piccolo
Jun 15, 2007·Nature Cell Biology·Hidekazu IiokaNoriyuki Kinoshita
Jul 10, 2002·Nature Cell Biology·Jason R JessenLilianna Solnica-Krezel
Sep 2, 2000·Development, Growth & Differentiation·A IshimuraM Maéno
Jan 10, 2001·Development, Growth & Differentiation·M Tada, J C Smith
Sep 29, 2001·Development, Growth & Differentiation·H TiedemannW Knöchel
May 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Christopher S CselenyiEthan Lee
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Soeren LienkampGerd Walz
Jun 23, 2010·The Journal of Biological Chemistry·Reiko SatowTesshi Yamada
Mar 19, 2009·Zebrafish·Masazumi Tada, Masatake Kai
Oct 8, 1999·Molecular Biology of the Cell·M W KlymkowskyY E Vourgourakis
Mar 14, 2008·Molecular Biology of the Cell·Nagatoki KinoshitaShigenobu Yonemura
Feb 28, 2002·The EMBO Journal·Rachel S DarkenPaul A Wilson
Dec 29, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R KellerP Skoglund
Sep 15, 1999·Genes & Development·K Itoh, S Y Sokol
Feb 4, 2011·Genes & Development·John B Wallingford, Brian Mitchell
May 13, 2010·Science Signaling·Kristin K JerniganEthan Lee
May 9, 2002·Molecular and Cellular Biology·Takayuki KadoyaAkira Kikuchi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.