Analysis of DNA Methylation at Specific Loci in Stool Samples Detects Colorectal Cancer and High-Grade Dysplasia in Patients With Inflammatory Bowel Disease

Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association
John B KisielDavid A Ahlquist

Abstract

Patients with inflammatory bowel diseases (IBDs), including ulcerative colitis and Crohn's disease, are at increased risk for colorectal cancer (CRC). Analyses of DNA methylation patterns in stool samples have been reported to detect CRC in patients with IBD. We sought to validate these findings in larger cohorts and assess the accuracy of analysis of DNA methylation patterns in stool for detection of CRC and high-grade dysplasia (HGD) normalized to methylation level at ZDHHC1. We obtained buffered, frozen stool samples from a US case-control study and from 2 European surveillance cohorts (referral or population based) of patients with chronic ulcerative colitis (n = 248), Crohn's disease (n = 82), indeterminate colitis (n = 2), or IBD with primary sclerosing cholangitis (n = 38). Stool samples were collected before bowel preparation for colonoscopy or at least 1 week after colonoscopy. Among the study samples, stools from individuals with IBD but without neoplasia were used as controls (n = 291). DNA was isolated from stool, exposed to bisulfite, and then assayed by multiplex quantitative allele-specific real-time target and signal amplification. We analyzed methylation levels of BMP3, NDRG4, VAV3, and SFMBT2 relative to the m...Continue Reading

Citations

Oct 19, 2019·World Journal of Clinical Cases·Yu-Xia Zhan, Guang-Hua Luo
Sep 19, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yi QinJohn B Kisiel
Jan 8, 2021·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·Mahir GachabayovRoberto Bergamaschi
Mar 23, 2021·The Journal of International Medical Research·Juan WangXiaoke Hao
May 1, 2021·Gastroenterology·Kristiina RajamäkiLauri A Aaltonen
Jul 3, 2021·Cancers·Mehmet YalchinAilsa Hart
Jun 14, 2021·The Lancet. Gastroenterology & Hepatology·Shahida DinIan D R Arnott
Jul 3, 2021·Oncology Reports·Can Kong, Tao Fu
Oct 2, 2021·Oncology Letters·Ruikai LiJianyong Zheng

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