Analysis of drug-protein interactions by high-performance affinity chromatography: interactions of sulfonylurea drugs with normal and glycated human serum albumin

Methods in Molecular Biology
Ryan MatsudaDavid S Hage

Abstract

High-performance affinity chromatography (HPAC) is a type of liquid chromatography that has seen growing use as a tool for the study of drug-protein interactions. This report describes how HPAC can be used to provide information on the number of binding sites, equilibrium constants, and changes in binding that can occur during drug-protein interactions. This approach will be illustrated through recent data that have been obtained by HPAC for the binding of sulfonylurea drugs and other solutes to the protein human serum albumin (HSA), and especially to forms of this protein that have been modified by non-enzymatic glycation. The theory and use of both frontal analysis and zonal elution competition studies in such work will be discussed. Various practical aspects of these experiments will be presented, as well as factors to consider in the extension of these methods to other drugs and proteins or additional types of biological interactions.

Citations

Jan 25, 2020·Electrophoresis·Hana NevídalováZdeněk Glatz
Sep 12, 2018·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pingyang TaoDavid S Hage
Jan 18, 2019·Journal of Pharmaceutical and Biomedical Analysis·Lucile LecasClaire Demesmay
Apr 30, 2017·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·James M KellyJohn W Babich

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