Analysis of dynamic protein carbonylation in rice embryo during germination through AP-SWATH

Proteomics
Hui ZhangPingfang Yang

Abstract

Seed germination is an important aspect of the plant life cycle, during which, reactive oxygen species (ROS) accumulate. The accumulation of ROS results in an increase in protein oxidation of which carbonylation is the most canonical one. However, there is insufficient information concerning protein oxidation, especially carbonylation and its contribution to seed germination. In this study, biotin hydrazide labeled chromatography combined with sequential window acquisition of all theoretical fragment ion spectra (SWATH) method was used to analyze the dynamic pattern of protein carbonylation in rice embryos during germination. A total of 1872 unique proteins were quantified, among which 288 carbonylated peptides corresponding to 144 proteins were determined based on the filtering through mass shifts of modified amino acids. In addition, 66 carbonylated proteins were further analyzed based on their carbonylation intensity in four stages of germination. These identified carbonylated proteins were mainly involved in maintaining the levels of ROS, abscisic acid and seed reserves. Remarkably, a peroxiredoxin was found with 23 unique carbonylated peptides, and the expression of which was consistent with its increased activity. This st...Continue Reading

References

Aug 15, 1997·The Journal of Biological Chemistry·B S Berlett, E R Stadtman
Jul 27, 1999·The Plant Journal : for Cell and Molecular Biology·R A StacyR B Aalen
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·S DukanT Nyström
Jan 25, 2002·IUBMB Life·S G RheeK Kim
Apr 30, 2002·Free Radical Biology & Medicine·Rodney L Levine
May 3, 2002·The Journal of Biological Chemistry·Tong-Shin ChangSue Goo Rhee
Sep 5, 2002·The Plant Journal : for Cell and Molecular Biology·Sarah L PritchardIan A Graham
Jul 1, 1997·The Plant Cell·J. D. Bewley
Feb 19, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Isabella Dalle-DonneRoberto Colombo
Dec 9, 2003·Amino Acids·J R RequenaE R Stadtman
May 24, 2005·Plant Physiology·Claudette JobDominique Job
Mar 23, 2007·The Plant Journal : for Cell and Molecular Biology·Krystyna OraczChristophe Bailly
Sep 13, 2007·Proteomics·Pingfang YangShihua Shen
Feb 9, 2008·Annual Review of Plant Biology·Ruth FinkelsteinCamille Steber
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Oct 6, 2009·PloS One·Etienne MaisonneuveSam Dukan
Jun 5, 2010·Journal of Proteome Research·Ashraf G Madian, Fred E Regnier
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Oct 30, 2010·Protein Science : a Publication of the Protein Society·Stephanie Hughes, Steffen P Graether
Jan 18, 2011·Journal of Proteome Research·María LinaresJosé M Bautista
May 24, 2011·Journal of Proteomics·Ian M MøllerR S P Rao
Jun 30, 2011·Plant, Cell & Environment·Gregorio Barba-EspínJosé Antonio Hernández
Sep 16, 2011·Proteomics·R Shyama Prasad Rao, Ian Max Møller
Jul 12, 2012·The Plant Journal : for Cell and Molecular Biology·Georgia TanouAthanassios Molassiotis
Oct 5, 2012·Proteomics·Imen LounifiGeorgia Tanou

❮ Previous
Next ❯

Citations

Mar 2, 2017·Analytical Chemistry·Juliane WeißerKeiryn L Bennett
Sep 10, 2019·Mass Spectrometry Reviews·Ana Margarida RodriguesCarla António
Jan 21, 2017·Frontiers in Plant Science·Enshun XuZhoufei Wang
Mar 2, 2017·Proteomes·Malgorzata CzarnaHanna Janska
Mar 16, 2018·Plant, Cell & Environment·Pascale SatourFrançoise Montrichard
Jul 25, 2017·Frontiers in Physiology·Brian McDonagh
Feb 21, 2019·Annual Review of Plant Biology·A Harvey MillarWaltraud X Schulze
Apr 9, 2021·Frontiers in Plant Science·Feng YuPingfang Yang
Oct 11, 2020·Trends in Biotechnology·Mo-Xian ChenFu-Yuan Zhu
Jun 1, 2021·Plant Cell Reports·Riyazuddin RiyazuddinRavi Gupta

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.