Analysis of EGFR, TGF-alpha, neu and c-myc in 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced mammary tumors using RT-PCR

Carcinogenesis
C D Davis, E G Snyderwine

Abstract

2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), a mutagen found in cooked meat, has been shown to induce mammary gland tumors in rats. Our laboratory recently observed that a high fat diet enhances the incidence and severity of PhIP-induced mammary gland cancer in rats. In the current study, reverse transcription followed by polymerase chain reaction amplification was used to determine whether EGFR, TGF-alpha, neu and c-myc are differentially expressed in PhIP-induced mammary gland tumors classified histologically as benign or malignant and to evaluate whether dietary fat intake influences the expression of these genes. Of 23 total PhIP-induced mammary tumors examined, 43%, 57% and 74% had increased expression of EGFR, TGF-alpha and neu mRNA respectively. Increased expression of these genes appeared to be consistently present in tumors displaying papillomatosis. In contrast, to the other three genes, c-myc mRNA levels were infrequently elevated. The percentage of dietary fat did not appear to influence the expression of EGFR, TGF-alpha or neu in either tumors or mammary gland from control rats. However, the levels of c-myc mRNA were 1.8- and 2.9-fold higher in the control mammary gland and benign PhIP-induced tumors res...Continue Reading

Citations

Oct 31, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S C PresnellG J Smith
Dec 16, 2006·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·E EscrichL Grau
Jan 8, 1999·Breast Cancer Research and Treatment·S J Roberts-Thomson, E G Snyderwine
May 23, 1998·Annual Review of Pharmacology and Toxicology·S H Safe
May 26, 2012·The Journal of Surgical Research·Erin E PerroneEric D Strauch
Sep 21, 2007·Molecular Nutrition & Food Research·Eduard EscrichMontserrat Solanas
Sep 22, 1999·Oncogene·G SourvinosD A Spandidos

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