Analysis of Ehrlichia ruminantium-specific T1/T2 responses during vaccination with a protective killed vaccine and challenge of goats

Parasite Immunology
Isabel EstevesP Totté

Abstract

Ehrlichia ruminantium is an obligate intracellular bacterium that causes heartwater in ruminants and for which T-cell-mediated immunity is believed to play an important role in protection. To better characterize protective cellular immunity, E. ruminantium-specific IFN-gamma and IL-4 recall responses in major T-cell subsets were analysed by flow cytometry during immunization of goats with a killed vaccine and following a virulent challenge. The killed vaccine elicited both CD8+ and CD4+ subsets to produce cytoplasmic IFN-gamma in the absence of IL-4, thus indicating a biased T1 response. The relative capacity of CD8+ T-cells to produce IFN-gamma was significantly higher than CD4+ T-cells but the final contribution of both subsets was comparable. Circulating ER-specific CD4 and CD8 effectors substantially decreased in numbers after the booster injection and could not be detected in most animals during challenge, which warrants further investigation in immune compartments other than blood. Since IFN-gamma inhibits the growth of the pathogen in target cells, the information provided in this study on E. ruminantium-specific T1 responses will be valuable to develop cellular tools for the identification of potential protective antigens.

References

Jan 1, 1991·Veterinary Immunology and Immunopathology·W I Morrison, W C Davis
Jan 1, 1991·Veterinary Immunology and Immunopathology·A Bensaid, M Hadam
Jan 1, 1995·Research in Veterinary Science·S M MahanA F Barbet
Dec 22, 1998·Veterinary Immunology and Immunopathology·V WeynantsJ J Letesson
Oct 3, 1999·Veterinary Immunology and Immunopathology·P TottéD McKeever
Apr 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M S Rolph, S H Kaufmann
May 16, 2002·Journal of Immunological Methods·Angelo MenaDale L Godson
Jul 10, 2002·Trends in Immunology·Shelley G Rhodes, Simon P Graham
May 7, 2004·Veterinary Immunology and Immunopathology·Isabel EstevesPhilippe Totté

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Citations

Oct 20, 2009·Veterinary Parasitology·Basil A Allsopp
May 3, 2005·Current Opinion in Infectious Diseases·Gary M Winslow, Constantine Bitsaktsis
Sep 9, 2010·Expert Review of Vaccines·Jere W McBride, David H Walker
Jun 27, 2013·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Inna V LinnikGeoff J M Parker
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Boondarika NamboopphaNattawooti Sthitmatee

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