Analysis of Enantioselective Biotransformations Using a Few Hundred Cells on an Integrated Microfluidic Chip

Journal of the American Chemical Society
Karin M KroneDetlev Belder

Abstract

The investigation of stereoselective biocatalytic transformations at a single-cell level is to date an unsolved challenge. Here, we report the development of an integrated microfluidic device which enables the analytical characterization of enantioselective reactions at nanoliter scale by combining whole-cell catalyzed on-chip syntheses, chiral microchip electrophoresis, and label-free detection of enantiomers by deep UV time-resolved fluorescence. Using Escherichia coli expressing recombinant Aspergillus niger epoxide hydrolase as the model enzyme for various enantioselective reactions, we evaluated the approach for downscaling the reaction to a few hundred cells. Our work is thus an important step toward the analysis of single-cell stereoselective biocatalysis.

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May 10, 2017·Biotechnology Journal·Pia GruberNicolas Szita
Feb 16, 2018·Angewandte Chemie·Xavier GarrabouDonald Hilvert
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Sep 20, 2020·Electrophoresis·Nan Lu, Jörg P Kutter
Feb 8, 2018·Current Opinion in Structural Biology·Hans Adrian BunzelDonald Hilvert

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