Analysis of Enterococcus faecalis in samples from Turkish patients with primary endodontic infections and failed endodontic treatment by real-time PCR SYBR green method.

Journal of Applied Oral Science : Revista FOB
Selcuk M OzbekAziz S Erdorgan

Abstract

The aims of this study were to investigate the presence of Enterococcus faecalis in primary endodontic infections and failed endodontic treatments using real-time PCR and to determine the statistical importance of the presence of E. faecalis in a Turkish population with endodontic infections. E. faecalis was investigated from 79 microbial samples collected from patients who were treated at the Endodontic Clinic of the Dental School of Atatürk University (Erzurum, Turkey). Microbial samples were taken from 43 patients (Group 1) with failed endodontic treatments and 36 patients (Group 2) with chronic apical periodontitis (primary endodontic infections). DNA was extracted from the samples by using a QIAamp DNA mini-kit and analyzed with real-time PCR SYBR Green. E. faecalis was detected in 41 out of 79 patients, suggesting that it exists in not less than 61% of all endodontic infections when the proportion test (z= -1.645, <x= 0.05) was applied. Real-time PCR SYBR Green allowed for the detection of E. faecalis in 32 out of 43 (74.4%) in Group 1, and in 9 out of 36 (25%) in Group 2. These results suggest that E. faecalis is a frequent isolate for endodontic infections in Turkish patients, and is more often associated with failed en...Continue Reading

References

Aug 11, 1991·Journal of Endodontics·J C Baumgartner, W A Falkler
Feb 1, 1995·Endodontics & Dental Traumatology·R WeigerC Löst
Feb 25, 1998·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·G SundqvistU Sjögren
May 11, 2001·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·H H HancockJ Moiseiwitsch
Aug 30, 2001·Journal of Clinical Microbiology·H J RolphJ Bagg
Sep 15, 2001·International Endodontic Journal·V PeciulieneM Haapasalo
May 3, 2002·International Endodontic Journal·M EvansD Figdor
May 23, 2002·Journal of Endodontics·José F SiqueiraA P Colombo
Aug 31, 2002·Journal of Clinical Microbiology·Ashraf F FouadJustin D Radolf
Jan 13, 2004·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·José F Siqueira, Isabela N Rôças
Mar 26, 2004·Nature Reviews. Microbiology·Didier RaoultMichel Drancourt
Apr 27, 2004·Journal of Endodontics·Isabela N RôçasKátia R N Santos
Dec 16, 2004·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Ashraf F FouadLarz S Spångberg
Jan 22, 2005·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·P N R NairJorge Vera
Apr 26, 2005·Archives of Oral Biology·C M SedgleyA Molander
Aug 1, 2006·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Brenda P F A GomesFrancisco José de Souza-Filho

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Citations

Feb 3, 2011·Clinical Oral Investigations·Sigrun EickAnton Sculean
May 18, 2011·Clinical Oral Investigations·Lamprini KarygianniAli Al-Ahmad
May 3, 2012·Medicina oral, patología oral y cirugía bucal·María-Teresa Arias-MolizCarmen-María Ferrer-Luque
Nov 3, 2012·ISRN Dentistry·Juliane Maria Guerreiro-TanomaruMário Tanomaru-Filho
Mar 29, 2014·Journal of Applied Oral Science : Revista FOB·Antonio Scalco FabrisMario Julio Avila-Campos
Mar 26, 2013·Dental Materials : Official Publication of the Academy of Dental Materials·Markus HeyderBernd W Sigusch
Nov 10, 2015·The Journal of Oral Implantology·Dennis Flanagan
Feb 3, 2015·Journal of Investigative and Clinical Dentistry·Raina T GergovaJulia Kamenoff
Jun 14, 2015·Journal of Endodontics·Ericka T PinheiroMárcia P A Mayer
Oct 27, 2010·Gut Microbes·Dawn A Israel, Richard M Peek
Mar 10, 2020·BioMed Research International·Mewan Salahalddin A AlrahmanKawa F Dizaye

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

SPSS ( Statistical Package for Social Science
SPSS

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