PMID: 2114535Jul 1, 1990Paper

Analysis of ENU-induced mutations at the Adh locus in Drosophila melanogaster

Mutation Research
N G FossettW R Lee

Abstract

N-Ethyl-N-nitrosourea (ENU) was used to induce mutations in the Drosophila melanogaster, alcohol dehydrogenase (Adh) gene. Flies were treated with ENU and mated to homozygous intragenic Adh null mutants; Adh null mutations were selected by exposure of the F1 generation to 1-penten-3-ol. Fourteen Adh null mutations were recovered which included 11 from spermatozoa, 2 from oocytes and 1 from a premeiotic spermatocyte. 2 mutations from spermatozoa and 1 of the mutations from oocytes were multilocus deficiencies which included the Adh locus as determined by complementation tests. The remaining 11 intragenic Adh null mutations were sequenced using the Sanger dideoxy method. One Adh null mutation induced in an oocyte was an AT to TA transversion and the mutation induced in a premeiotic spermatocyte was a GC to AT transition, both of which resulted in a single amino acid substitution. The 11 null mutations induced in spermatozoa were a data set in which both the dose of ENU and the treated germ-cell stage were held constant; therefore, only these 11 mutations were used to calculate the mutation frequency and compare the mutations at the Adh locus with those recovered in other studies. The dose of ENU induced a sex-linked recessive let...Continue Reading

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Citations

Aug 1, 1995·Mutation Research·E W Vogel, A T Natarajan
Oct 1, 1993·Environmental Health Perspectives·J Favor
May 9, 1995·Biochemistry·H JörnvallD Ghosh
Dec 24, 2010·PLoS Biology·Duarte MesquitaMarco Milán
Jan 1, 1995·Environmental and Molecular Mutagenesis·R A MittelstaedtR H Heflich
Jan 1, 1991·Environmental and Molecular Mutagenesis·J MahmoudW R Lee
Nov 8, 2002·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·Gabriele StummMichael Nehls
Jan 5, 2020·G3 : Genes - Genomes - Genetics·Rafael Hoyos-ManchadoVíctor A Tallada

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