PMID: 9426906Aug 1, 1997Paper

Analysis of epidemic Pseudomonas aeruginosa isolates by isoelectric focusing of pyoverdine and RAPD-PCR: modern tools for an integrated anti-nosocomial infection strategy in burn wound centres

Burns : Journal of the International Society for Burn Injuries
D De VosPierre Cornelis

Abstract

The Gram-negative bacterium Pseudomonas aeruginosa is an important life-threatening nosocomial pathogen in burn units. In this study we analysed epidemic P. aeruginosa isolates from patients and their hospital environment using two new molecular techniques in order to establish strain relatedness for epidemiological purposes. One technique was pyoverdine typing by isoelectric focusing (PVD-IEF) and the other was a genomic PCR-based fingerprinting technique called random amplification of polymorphic DNA actually referred to as RAPD-PCR. The described short epidemic (6 weeks) included 37 consecutive isolates from 9 different patients as well as two environmental isolates recovered, at the same time, from one of the hydrotherapy facilities. Only two of the three known pyoverdine types of P. aeruginosa could be found. Type I was absent while type II represented 49 per cent and type III, 51 per cent of the isolates. The two consecutive isolates from the environment were both of type III. The RAPD-PCR fingerprinting discriminated four patterns. Profile 1 represented 60 per cent; profile 2, 34 per cent; and profiles 3 and 4 only 3 per cent of the isolates respectively. The environmental isolates also had a RAPD-PCR 1 profile, arguing ...Continue Reading

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Citations

Mar 17, 2004·Diagnostic Microbiology and Infectious Disease·Jiunn Jong WuTsung Chain Chang
Feb 28, 2001·Burns : Journal of the International Society for Burn Injuries·M W DouglasT Gottlieb
Jun 30, 2000·Biochemical and Biophysical Research Communications·M J Wilson, I L Lamont
Jul 19, 2014·Environmental Science and Pollution Research International·Ana Flavia Tonelli FernandesEliana Guedes Stehling
Jul 25, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Vinicius Vicente MartinsEliana Guedes Stehling
Jun 11, 2005·Environmental Microbiology·Jean-Paul PirnayPierre Cornelis
Aug 12, 2014·Journal of Medical Microbiology·Greta FerrantiAnnalisa Bargellini
Dec 27, 2013·The Science of the Total Environment·André Pitondo-SilvaEliana Guedes Stehling
Jun 19, 2013·Burns : Journal of the International Society for Burn Injuries·Fatemeh NanvazadehNajmeh Parhizgari
Jun 21, 2014·Annals of Surgery·Shuangchun YanLaurence G Rahme
Aug 21, 2002·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Geir BukholmNils Smith-Erichsen
May 21, 2016·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·André Pitondo-SilvaEliana Guedes Stehling
Jul 13, 2017·BMC Infectious Diseases·Kavinda TisseraDulmini Nanayakkara

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