Analysis of epinine and its metabolites in man after oral administration of its pro-drug ibopamine using high-performance liquid chromatography with electrochemical detection

Journal of Chromatography
R GiffordJ A Ziemniak

Abstract

Ibopamine (N-methyldopamine O,O'-diisobutyrol ester, hydrochloride) is an ester prodrug of epinine. Epinine is a cardiovascular agent used in congestive heart failure because of its dopaminergic and adrenoreceptor agonist properties. Quantitative analytical methods, using high-performance liquid chromatography coupled with electrochemical detection, were developed for the determination of epinine and its known metabolites in biological media. Epinine was extracted from human plasma and urine via an alumina adsorption procedure; this procedure was also used to estimate epinine conjugates after prior enzymatic hydrolysis. Penicillamine was added to the incubation mixture to inhibit isoquinoline production. Urinary dihydroxyphenylacetic acid levels were obtained using the same alumina adsorption procedure, while a separate analytical procedure utilizing a direct high-performance liquid chromatographic analysis of samples was developed for homovanillic acid and its conjugates. Coefficients of variation for all the assays were below 8%. These methods were used to study the pharmacokinetics and metabolic fate of epinine after oral administration of ibopamine to healthy volunteers.

References

Jul 1, 1977·Journal of Chromatography·L J FeliceP T Kissinger
Jan 1, 1985·British Journal of Clinical Pharmacology·A Racine-Poon, J Moppert
Dec 1, 1971·Clinica Chimica Acta; International Journal of Clinical Chemistry·E R Smith, H Weile-Malherbe
Jan 1, 1984·European Journal of Clinical Pharmacology·G C ReffoC Forattini

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Citations

Mar 5, 1993·Journal of Chromatography·M KajitaT Nagatsu

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