PMID: 8614619Apr 1, 1996Paper

Analysis of eukaryotic mRNA structures directing cotranslational incorporation of selenocysteine

Nucleic Acids Research
H KollmusJ E McCarthy

Abstract

Translation of an mRNA encoding a selenoprotein requires that at least one UGA codon in the reading frame is recoded as a site for the insertion of selenocysteine. In eukaryotes, the termination codon recoding event is directed by a cis-acting signal element located in the 3' untranslated region of the gene. This 'selenocysteine insertion sequence' (SECIS) comprises conserved sequences in a region of extensive base-pairing. In order to study the structure-function relationships of the SECIS structure, we have applied a newly developed reporter gene system which allows analysis of stop codon suppression in animal cell lines. This system obviates the need for enzymatic or immunological estimation of selenoprotein synthesis, relying instead on the simple quantification of translational readthrough from the lacZ gene into the luciferase gene. The 3'-UTR of the phospholipid hydroperoxide glutathione peroxidase (PHGPx) gene was shown to contain a highly active SECIS element. Mutations in the base-paired sequences of other SECIS elements were used to analyse the significance of primary structure, secondary structure and pairing stability in the stem regions. The results demonstrate that the exact sequences of the paired nucleotides ar...Continue Reading

References

Sep 18, 1992·Science·R F GestelandJ F Atkins
Mar 1, 1991·Molecular Microbiology·A BöckF Zinoni
Dec 1, 1991·Trends in Biochemical Sciences·A BöckC Baron
Jan 1, 1990·Annual Review of Biochemistry·T C Stadtman
May 15, 1973·FEBS Letters·L FloheH H Schock
Jan 1, 1966·Cold Spring Harbor Symposia on Quantitative Biology·M NirenbergF Anderson
Feb 1, 1984·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W A GünzlerL Flohé
Jan 1, 1995·Annual Review of Biochemistry·D E Draper
May 1, 1995·Trends in Biochemical Sciences·J E McCarthy, H Kollmus
Nov 1, 1993·Biochemical Society Transactions·M J Berry, P R Larsen
Jul 1, 1993·Trends in Biochemical Sciences·M J Gait, J Karn

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Citations

Dec 28, 1999·BioFactors·K Wingler, R Brigelius-Flohé
Jul 2, 2003·Neurotoxicity Research·B H Juurlink
May 29, 2003·Journal of Insect Physiology·D LiO Schmidt
Apr 5, 2007·Nature Protocols·Md Talat Nasim, Ian C Eperon
Jul 2, 2004·The Journal of Biological Chemistry·Anupama MehtaPaul R Copeland
May 15, 2002·Antioxidants & Redox Signaling·Roderick C McKenzieGeoffrey J Beckett
Jul 25, 2000·Nucleic Acids Research·D FagegaltierA Krol
Nov 25, 2004·Nucleic Acids Research·Jonathan L Jacobs, Jonathan D Dinman
Sep 6, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mitsuko Hirosawa-TakamoriHerbert Jäckle
Apr 15, 2004·Genes & Development·Jeremy R SanfordJavier F Cáceres
Jun 23, 2004·RNA·Ruth R Jameson, Alan M Diamond
Apr 6, 2012·PloS One·Marco MariottiVadim N Gladyshev
Apr 3, 2014·PloS One·Emily N ReinkeAlan M Diamond
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·L ZhaoE W Taylor
Nov 8, 2002·Proceedings of the National Academy of Sciences of the United States of America·Dieter MaierAnette Preiss
Jan 3, 2012·The Journal of Nutritional Biochemistry·Shuvojit BanerjeeCharles B Foster
Apr 11, 2009·Biochimica Et Biophysica Acta·Leopold Flohé
Mar 31, 2009·Biochimica Et Biophysica Acta·Lutz Schomburg, Ulrich Schweizer
Dec 5, 2006·Molecular Microbiology·David Gordon LongstaffJoseph Adrian Krzycki
Feb 13, 2007·Archives of Biochemistry and Biophysics·L K CrosleyJ R Arthur
Apr 5, 2014·Metallomics : Integrated Biometal Science·Niels-Peter BeckerLutz Schomburg
Sep 11, 2014·Human Genetics·Mariana Martins RibeiroSergio Roberto Peres Line
Nov 26, 1999·Human Mutation·B GottliebM A Trifiro
Feb 29, 2000·The Journal of Biological Chemistry·R M TujebajevaM J Berry
Dec 23, 1999·The Journal of Biological Chemistry·A LescureA Krol

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