Analysis of expressed and non-expressed IGK locus rearrangements in chronic lymphocytic leukemia.

Molecular Medicine
Chrysoula BelessiNikolaos Laoutaris

Abstract

Immunoglobulin kappa (IGK) locus rearrangements were analyzed in parallel on cDNA/genomic DNA in 188 kappa- and 103 lambda-chronic lymphocytic leukemia (CLL) cases. IGKV-KDE and IGKJ-C-intron-KDE rearrangements were also analyzed on genomic DNA. In kappa-CLL, only 3 of 188 cases carried double in-frame IGKV-J transcripts: in such cases, the possibility that leukemic cells expressed more than one kappa chain cannot be excluded. Twenty-eight kappa-CLL cases also carried nonexpressed (nontranscribed and/or out-of-frame) IGKV-J rearrangements. Taking IGKV-J, IGKV-KDE, and IGKJ-C-intron-KDE rearrangements together, 38% of kappa-CLL cases carried biallelic IGK locus rearrangements. In lambda-CLL, 69 IGKV-J rearrangements were detected in 64 of 103 cases (62%); 24 rearrangements (38.2%) were in-frame. Four cases carried in-frame IGKV-J transcripts but retained monotypic light-chain expression, suggesting posttranscriptional regulation of allelic exclusion. In all, taking IGKV-J, IGKV-KDE, and IGKJ-C-intron-KDE rearrangements together, 97% of lambda-CLL cases had at least 1 rearranged IGK allele, in keeping with normal cells. IG repertoire comparisons in kappa- versus lambda-CLL revealed that CLL precursor cells tried many rearrangemen...Continue Reading

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Citations

Feb 6, 2013·Nature Reviews. Rheumatology·Sebastien ViatteSoumya Raychaudhuri
Dec 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Andrea ChoeArthur S Edison
Mar 14, 2007·Anti-cancer Drugs·Claude Monneret
Jan 10, 2012·Current Opinion in Hematology·Francesco PassamontiDomenica Caramazza
Aug 26, 2014·Jundishapur Journal of Microbiology·Aida GholoobiZahra Meshkat

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