Analysis of expressed sequence tags from Rhynchosciara americana salivary glands

Insect Molecular Biology
F SivieroR V Santelli

Abstract

The diptera Rhynchosciara americana (sciaridae) is an important model organism in polyteny and gene amplification research, but up to now a limited amount of data regarding DNA sequences and molecular aspects of this species is available. Considering the importance of going further on the DNA puffs biological meaning, we proposed to generate EST sequences from a DNA library constructed from salivary glands. After their categorization in gene ontology terms, they were used to construct an 'electronic Northern' that represents a general view of the salivary gland metabolic status in an important phase of larval development: the spinning of communal cocoon. In this phase occurs the last polytene DNA replication cycle concomitantly with the specific loci amplification related to protein secretion.

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Citations

Apr 25, 2012·Development Genes and Evolution·Paula Rezende-TeixeiraGláucia Maria Machado-Santelli
Jun 6, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Paula Rezende-TeixeiraGláucia M Machado-Santelli
Aug 21, 2007·Genetica·Paula Rezende-TeixeiraGláucia M Machado-Santelli
May 8, 2010·Genetics and Molecular Research : GMR·P Rezende-TeixeiraG M Machado-Santelli
Sep 22, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Alexandre de AndradeGlaucia Maria Machado-Santelli
May 22, 2009·Annals of the New York Academy of Sciences·R J Weaver, N Audsley
Jun 20, 2014·Arthropod Structure & Development·Amanda Dos Santos BrandãoGláucia Maria Machado-Santelli
May 15, 2016·Genesis : the Journal of Genetics and Development·Claudio Roberto SimonNadia Monesi
Oct 12, 2016·Genetics and Molecular Biology·Fábio SivieroGlaucia Maria Machado-Santelli
Nov 11, 2021·Open Biology·Jorge Henrique NevesGlaucia Maria Machado-Santelli

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