Abstract
The adherent chicken cell line, MDCC-MSB1-41C, was highly transplantable and metastatic in vivo, compared with the parental non-adherent cell line MDCC-MSB1 from Marek's disease (MD) lymphoblastoid tumor. For clarification of differences in extracellular matrix proteins in MSB1-41C and MSB1 cells, examination was made of various components of extracellular matrix proteins. A detachment experiment indicated the protein(s) recognizing the Arg-Gly-Asp (RGD) sequence, the minimum structure required for recognition by the cell-surface receptors, is essential for the adherent character. Immunoblot assay using antibodies showed increased expression of fibronectin, fibronectin receptors, and vinculin on MSB1-41C cell lines. RGD-directed integrins mediate important cell-cell adhesive interaction and these interactions with extracellular matrix proteins may thus possibly be requisite for migration, proliferation and metastatic dissemination of MDCC-MSB1-41C cells. The RGD-containing peptide in the culture medium could cause detachment of cultured adhesive lymphoid leukosis LSCC-1104X5 cells from the dish too.
References
Apr 1, 1992·Journal of Leukocyte Biology·C A OwenR A Stockley
Oct 1, 1992·The Journal of Cell Biology·J L Rodríguez FernándezA Ben-Ze'ev
Jul 1, 1990·Electrophoresis·H Hirano, T Watanabe
Feb 28, 1986·Cell·E Ruoslahti, M D Pierschbacher
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P M Cardarelli, M D Pierschbacher
Jun 1, 1985·The Journal of Cell Biology·E G HaymanE Ruoslahti
Jan 1, 1987·Annual Review of Cell Biology·C A Buck, A F Horwitz
Dec 1, 1985·The Journal of Cell Biology·A HorwitzC Buck
Jan 1, 1988·Annual Review of Biochemistry·E Ruoslahti
Oct 1, 1988·The Journal of Cell Biology·B GumbinerA Grimaldi
Jan 1, 1987·Kardiologiia·Iu B BelousovE P Panchenko
Jan 1, 1985·Journal of Cellular Biochemistry·M D PierschbacherE Ruoslahti
Jul 1, 1984·Journal of Virology·T KoyamaS Kawai
Jan 1, 1982·Cold Spring Harbor Symposia on Quantitative Biology·B Geiger
Sep 1, 1993·The Journal of Cell Biology·J L Rodríguez FernándezA Ben-Ze'ev
Jul 1, 1980·Avian Pathology : Journal of the W.V.P.A·W OkazakiD Ewert
Citations
Jul 16, 2008·Chembiochem : a European Journal of Chemical Biology·Sander S van BerkelFloris P J T Rutjes
Apr 20, 2007·Proteomics·Joram J Buza, Shane C Burgess
May 16, 2002·Bioconjugate Chemistry·Zi-Fen SuDonald J Hnatowich