Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients

International Journal of Molecular Sciences
Shuji KagotaKazuhisa Uchiyama

Abstract

Extracellular vesicles (EVs) are secretory membrane vesicles containing lipids, proteins, and nucleic acids; they function in intercellular transport by delivering their components to recipient cells. EVs are observed in various body fluids, i.e., blood, saliva, urine, amniotic fluid, and ascites. EVs secreted from cancer cells play important roles in the formation of their environment, including fibrosis, angiogenesis, evasion of immune surveillance, and even metastasis. However, EVs in gastric juice (GJ-EVs) have been largely unexplored. In this study, we sought to clarify the existence of GJ-EVs derived from gastric cancer patients. GJ-EVs were isolated by the ultracentrifuge method combined with our own preprocessing from gastric cancer (GC) patients. We verified GJ-EVs by morphological experiments, i.e., nanoparticle tracking system analysis and electron microscopy. In addition, protein and microRNA markers of EVs were examined by Western blotting analysis, Bioanalyzer, or quantitative reverse transcription polymerase chain reaction. GJ-EVs were found to promote the proliferation of normal fibroblast cells. Our findings suggest that isolates from the GJ of GC patients contain EVs and imply that GJ-EVs partially affect thei...Continue Reading

References

Nov 1, 1985·Digestive Diseases and Sciences·A Allen, N J Carroll
Jul 6, 1981·Biochimica Et Biophysica Acta·E G TramsU Heine
Mar 1, 1996·The Journal of Experimental Medicine·G RaposoH J Geuze
Aug 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Trairak PisitkunMark A Knepper
May 24, 2005·International Immunology·Marie-Pierre CabyChristian Bonnerot
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Charlotte AdmyreSusanne Gabrielsson
Mar 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·D Michiel PegtelJaap M Middeldorp
Jun 10, 2010·Journal of Experimental & Clinical Cancer Research : CR·Kangkang ZhiJianwei Bi
Jan 7, 2011·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Masakazu Yashiro, Kosei Hirakawa
Jan 8, 2011·Biological & Pharmaceutical Bulletin·Yuko OgawaRyohei Yanoshita
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Apr 9, 2014·Nature Communications·Yusuke YoshiokaTakahiro Ochiya
Apr 24, 2015·Journal of Neuro-oncology·Johnny C AkersClark C Chen
Sep 12, 2015·World Journal of Gastroenterology : WJG·Yu YanRui-Fen Wang
Jan 23, 2016·Cancer Science·Yu FujitaTakahiro Ochiya
Jun 23, 2016·Journal of Extracellular Vesicles·Zoraida AndreuMaría Yáñez-Mó
Sep 2, 2016·Cellular and Molecular Life Sciences : CMLS·Yutaka NaitoTakahiro Ochiya
Oct 19, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jingyun LeeSunil Hwang
Sep 24, 2016·Scientific Reports·Wataru NakaiRikinari Hanayama
Dec 14, 2016·Cancer Cell·Annette BeckerDavid Lyden
Jul 12, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Haruka ShinoharaYukihiro Akao
Aug 23, 2017·Methods in Molecular Biology·Richard Lobb, Andreas Möller
Oct 8, 2017·Biochemical and Biophysical Research Communications·Ning WangXin Liu
Jan 10, 2018·Molecular Carcinogenesis·Kohei TaniguchiYukihiro Akao
Feb 6, 2018·Cancer Biomarkers : Section a of Disease Markers·Jianjun WangGuangxin Li
Jun 12, 2018·Journal of Extracellular Vesicles·Dominik BuschmannMarlene Reithmair

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Citations

Jan 25, 2020·Digestive Diseases and Sciences·Fumio TanakaYasuhiro Fujiwara
May 1, 2021·International Journal of Molecular Sciences·Yuko ItoKazuhisa Uchiyama
Sep 1, 2021·Advanced Science·Li MinShutao Wang

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Methods Mentioned

BETA
transmission electron microscopy
Protein assay

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