Analysis of fifteen estrogen metabolites using packed column supercritical fluid chromatography-mass spectrometry

Analytical Chemistry
Xia XuHaleem J Issaq

Abstract

Packed column supercritical fluid chromatography with tandem mass spectrometry was used for the separation of estrone, estradiol, estriol, 16-epiestriol, 17-epiestriol, 16-ketoestradiol, 16alpha-hydroxyestrone, 2-methoxyestrone, 4-methoxyestrone, 2-hydroxyestrone-3-methyl ether, 2-methoxyestradiol, 4-methoxyestradiol, 2-hydroxyestrone, 4-hydroxyestrone, and 2-hydroxyestradiol. A gradient of methanol in carbon dioxide (0-30% methanol in 15 min, 2% change/min) at a flow rate of 2 mL/min and cyanopropyl silica column connected in series with a diol column, both 2.1 mm i.d. x 150 mm long, packed with 5-mum spherical silica-based particles, resulted in the separation and quantification of all 15 estrogens in less than 10 min. The limit of detection (LOD) and limit of quantitation (LOQ) of this pSFC MS/MS method was determined to be 0.5 (S/N = 3), and 5 pg, respectively. Compared with RP-HPLC MS analysis of the same mixture in terms of speed of analysis and sensitivity, pSFC MS is much faster, 10 versus 70 min, with comparable LOD and LOQ.

References

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Citations

Jul 22, 2008·Journal of the American Society for Mass Spectrometry·Tai-Chia ChiuHuan-Tsung Chang
Nov 19, 2010·Bioanalysis·Atsuki MatsubaraTakeshi Bamba
Nov 19, 2010·Bioanalysis·Angel RíosFernando de Andrés
Jan 16, 2013·Journal of Bioscience and Bioengineering·Muneo Saito
Mar 3, 2009·Annals of the New York Academy of Sciences·Daniel W Sepkovic, H Leon Bradlow
Oct 19, 2013·Chemosphere·Nadine GoeppertBrian Berkowitz
Aug 1, 2014·Bioanalysis·Kaori TaguchiTakeshi Bamba
Jan 22, 2015·Journal of Chromatography. a·Eric Lesellier, Caroline West
Mar 20, 2016·Fertility and Sterility·Soledad HenríquezLuigi Devoto
Dec 1, 2006·Journal of Mass Spectrometry : JMS
Mar 19, 2008·Journal of Separation Science·Haleem J IssaqTimothy D Veenstra
Mar 28, 2008·Journal of Separation Science·Eric AbbottHaleem J Issaq
Mar 28, 2008·Journal of Separation Science·Fangbiao Li, Yunsheng Hsieh
Apr 23, 2008·Analytical Chemistry·Larry T Taylor
May 1, 2021·Biomolecules·Michal KaletaMiroslav Strnad
Sep 25, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Nicholas A MoringoChristy F Landes

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