Analysis of filamentous process induction and nuclear localization properties of the HSV-2 serine/threonine kinase Us3.

Virology
Renée L FinnenBruce W Banfield

Abstract

The Us3 serine/threonine kinase encoded by all alphaherpesviruses performs several important functions during virus multiplication. For example, expression of pseudorabies virus (PRV) Us3 causes reorganization of the actin cytoskeleton into filamentous processes (FPs) that promote cell-to-cell spread of virus infection. PRV Us3-induced FP formation requires Us3 kinase activity. To determine whether these characteristics were shared by HSV-2 Us3, expression plasmids for wild type (WT) and kinase dead (KD) Us3 variants were constructed. Expression of WT Us3 resulted in robust FP formation whereas expression of the KD Us3 variants did not. In the course of these experiments we noted that KD/K220 mutant Us3s were excluded from the nucleus in comparison to WT or KD/D305A Us3, prompting us to investigate Us3 nuclear shuttling properties. Herein we describe determinants of HSV-2 Us3-induced FP formation and present evidence for the presence of a leucine-rich nuclear export signal within HSV-2 Us3.

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Citations

Nov 19, 2010·Journal of Virology·Thary JacobHerman W Favoreel
Oct 14, 2011·Viruses·Céline Van den Broeke, Herman W Favoreel
Oct 14, 2011·Viruses·Sofia V ZaichickGregory A Smith
May 25, 2012·Journal of Virology·Renée L FinnenBruce W Banfield
Aug 18, 2012·PloS One·Valerie Le Sage, Bruce W Banfield
Mar 15, 2013·Journal of Virology·Valerie Le SageBruce W Banfield
Nov 28, 2015·Viruses·Ian B HogueLynn W Enquist
Jun 15, 2011·Veterinary Microbiology·María Fátima LadelfaGabriela Calamante
Jan 15, 2010·Trends in Cell Biology·Celine Van den BroekeHerman W Favoreel
Oct 15, 2010·The Journal of General Virology·M J Deruelle, H W Favoreel
Mar 4, 2010·Veterinary Microbiology·Herman W FavoreelNick De Regge
Jul 12, 2011·Virology·Renée L FinnenBruce W Banfield
Apr 3, 2014·Small GTPases·Céline Van den BroekeHerman W Favoreel
Feb 14, 2018·Viruses·Christopher E DenesRussell J Diefenbach
Jan 17, 2020·Journal of Virology·Julia SehlThomas C Mettenleiter
Feb 7, 2020·Journal of Virology·Robert J J JansensHerman W Favoreel
Apr 18, 2015·The Journal of General Virology·Thary JacobHerman W Favoreel
Apr 29, 2020·Nature Communications·Adam W WhisnantLars Dölken
Aug 23, 2020·International Journal of Molecular Sciences·Imran Ahmad, Duncan W Wilson

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