Analysis of flash-induced FTIR difference spectra of the S-state cycle in the photosynthetic water-oxidizing complex by uniform 15N and 13C isotope labeling

Biochemistry
Takumi Noguchi, Miwa Sugiura

Abstract

Protein bands in flash-induced Fourier transform infrared (FTIR) difference spectra of the S-state cycle of photosynthetic water oxidation were analyzed by uniform (15)N and (13)C isotopic labeling of photosystem II (PS II). The difference spectra upon first- to fourth-flash illumination were obtained with hydrated (for the 1800-1200 cm(-)(1) region) or deuterated (for the 3500-3100 cm(-)(1) region) films of unlabeled, (15)N-labeled, and (13)C-labeled PS II core complexes from Thermosynechococcus elongatus. Shifts of band frequencies upon (15)N and (13)C labeling provided the assignments of major peaks in the regions of 3450-3250 and 1700-1630 cm(-)(1) to the NH stretches and amide I modes of polypeptide backbones, respectively, and the assignments of some of the peaks in the 1600-1500 cm(-)(1) region to the amide II modes of backbones. Other prominent peaks in the latter region and most of the peaks in the 1450-1300 cm(-)(1) region exhibited large downshifts upon (13)C labeling but were unchanged by (15)N labeling, and hence assigned to the asymmetric and symmetric COO(-) stretching vibrations, respectively, of carboxylate groups in Glu, Asp, or the C-terminus. Peak positions corresponded well with each other among the first- ...Continue Reading

References

Oct 16, 1992·Biochimica Et Biophysica Acta·R J Debus
Mar 26, 1998·Analytical Biochemistry·K RahmelowT Ackermann
Nov 7, 1996·Chemical Reviews·Vittal K. YachandraMelvin P. Klein
Jan 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nobuo Kamiya, Jian-Ren Shen

❮ Previous
Next ❯

Citations

Apr 22, 2008·Photosynthesis Research·Alain BoussacMiwa Sugiura
Jun 11, 2009·Photosynthesis Research·Catherine Berthomieu, Rainer Hienerwadel
Sep 26, 2009·Journal of the American Chemical Society·Ruth GrossRolf Diller
Apr 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Brandon C Polander, Bridgette A Barry
Oct 24, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Melodie A StricklerRichard J Debus
Oct 30, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Takumi Noguchi
Dec 31, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yuki KatoTakumi Noguchi
Jul 29, 2006·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Tatsunori Okubo, Takumi Noguchi
Jun 30, 2005·Biophysical Journal·Bridgette A BarryDavid L Jenson
Sep 24, 2015·Physical Chemistry Chemical Physics : PCCP·Haochen Ke, James M Lisy
Jun 20, 2006·Biophysical Journal·Antonio De RisoBridgette A Barry
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Shin Nakamura, Takumi Noguchi
Jun 22, 2017·Journal of the American Chemical Society·Shin Nakamura, Takumi Noguchi
May 3, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Mingxin LvYue Liu
Nov 16, 2004·The Journal of Biological Chemistry·Yukihiro KimuraTaka-aki Ono
Jan 26, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Hongbo YuShenghu Zhou
Mar 24, 2020·Chemical Reviews·Victor A Lorenz-Fonfria

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.