Analysis of fluoxetine and norfluoxetine in human plasma by liquid-phase microextraction and injection port derivatization GC-MS

Journal of Pharmaceutical and Biomedical Analysis
Antônio Felipe Felicioni OliveiraAlvaro José Dos Santos-Neto

Abstract

A two-phase liquid phase microextraction using a hollow fiber combined with injection port derivatization and gas chromatographic analysis was developed for extracting and detecting fluoxetine (FLU) and norfluoxetine (NOR) in human plasma. Simultaneous extraction in a multiple tube shaker was used and, afterward, the organic phase was simply injected together with the derivatizing agent n-methyl-bis(trifluoroacetamide) (MBTFA). Factors influencing injection port derivatization, and several extraction parameters were optimized. Under optimal conditions the proposed method provided linearity between 10 and 500ngmL(-1) (R(2)=0.9973) for FLU, and between 15 and 500ngmL(-1) (R(2)=0.9972) for NOR. Intra-assay precision (RSD) between 4.8 and 13.1% and inter-assay between 5.4 and 14.2% were obtained, with detection and quantification limits of 3 and 10ngmL(-1), and of 5 and 15ngmL(-1) for FLU and NOR, respectively, using selected ion monitoring mode. Selectivity, short term stability and extraction efficiency were also evaluated. This method was simple, cheap, and environmentally friendly, yielding significant reduction of solvents and derivatizing agent consumption. The method was successfully applied to the analysis of samples from 5...Continue Reading

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Citations

Mar 5, 2016·Luminescence : the Journal of Biological and Chemical Luminescence·Ali Lotfi, Jamshid L Manzoori
Jan 16, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiangjun QiuXue-sun Huang
Sep 25, 2018·Biomedical Chromatography : BMC·Letícia Aparecida MarquesSuzana Lucy Nixdorf
Apr 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ruiqin ZhuChuixiu Huang
Jul 2, 2021·Analytical and Bioanalytical Chemistry·Noemí Molina-FernándezJon Sanz-Landaluze

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