Analysis of force curves obtained on the live cell membrane using chemically modified AFM probes

Ultramicroscopy
Rehana AfrinAtsushi Ikai

Abstract

Force curves were obtained on the live cell surface using an atomic force microscope mounted with a modified tip with the bifunctional covalent crosslinker, disuccinimidyl suberate, which forms a covalent bond with amino-bearing molecules on the cell surface. A ramp delay time of 1.0 s was introduced before the start of the retraction regime of the force curve to increase the stationary reaction time between the crosslinkers on the tip and the amino groups on the cell surface. While live cell surface responses to forced contact with a non-functionalized tip rarely showed evidence of tip-cell interaction, those obtained with modified tips gave clear indication of prolonged adhesion which was terminated by a single step release of the tip to its neutral position. Under the given experimental conditions of this work, 58% of a total of 198 force curves gave only one jump and 70% of those with one jump gave the final rupture force of 0.45+/0.22 [corrected] nN. The result emphasized the uniqueness of the observed mechanical response of the cell surface when probed with chemically modified tips.

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Citations

Mar 15, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Atsushi Ikai
Dec 1, 2009·Nature Materials·Dong-Hyun KimValentyn Novosad
Mar 28, 2008·European Biophysics Journal : EBJ·L SirghiF Rossi
Dec 25, 2007·Analytical and Bioanalytical Chemistry·Christian BudichVolker Deckert
May 11, 2005·Biophysical Chemistry·Atsushi Ikai
Mar 10, 2010·Ultramicroscopy·Song Cui, Yeng Chai Soh
Feb 19, 2015·Nanoscale·Musashi TakenakaAkihiko Kondo
Jan 26, 2006·IEEE Transactions on Nanobioscience·Pau GorostizaEhud Y Isacoff
Sep 9, 2017·Scientific Reports·De Wei WongWen Siang Lew
Nov 22, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Antoine JégouChristine Gourier
Nov 21, 2018·ACS Applied Materials & Interfaces·Yuqiang XiangYonggang Hu
May 3, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Li-Chong Xu, Bruce E Logan

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