Analysis of forward and backward Second Harmonic Generation images to probe the nanoscale structure of collagen within bone and cartilage

Journal of Biophotonics
Marie-Andree HouleFrancois Légaré

Abstract

Collagen ultrastructure plays a central role in the function of a wide range of connective tissues. Studying collagen structure at the microscopic scale is therefore of considerable interest to understand the mechanisms of tissue pathologies. Here, we use second harmonic generation microscopy to characterize collagen structure within bone and articular cartilage in human knees. We analyze the intensity dependence on polarization and discuss the differences between Forward and Backward images in both tissues. Focusing on articular cartilage, we observe an increase in Forward/Backward ratio from the cartilage surface to the bone. Coupling these results to numerical simulations reveals the evolution of collagen fibril diameter and spatial organization as a function of depth within cartilage.

References

Nov 1, 1975·Arthritis and Rheumatism·J M Lane, C Weiss
Aug 1, 1992·The Journal of Pathology·W S HwangG N Hughes
Oct 1, 1990·Current Opinion in Cell Biology·M K Gordon, B R Olsen
Jan 1, 1995·Annual Review of Biochemistry·D J Prockop, K I Kivirikko
Oct 1, 1995·Current Opinion in Cell Biology·B R Olsen
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y GuoR R Alfano
Oct 2, 2001·Current Opinion in Chemical Biology·R M WilliamsW W Webb
May 23, 2002·Biophysical Journal·Patrick StollerAlexander M Rubenchik
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Aikaterini ZoumiBruce J Tromberg
May 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Warren R ZipfelWatt W Webb
Mar 23, 2005·Osteoarthritis and Cartilage·Alvin T YehGeorge M Peavy
Apr 24, 2007·Biophysical Journal·François LégaréBjorn R Olsen
Jun 7, 2007·Journal of Cell Science·Karl E KadlerRaymond P Boot-Handford
Nov 22, 2008·Journal of Biomedical Optics·Jessica C MansfieldSteve J Matcher
Jun 9, 2009·Micron : the International Research and Review Journal for Microscopy·Ali Hussain Reshak
Apr 2, 2007·Optics Express·M StruplerM-C Schanne-Klein
Jun 11, 2007·Optics Express·Christian P PfefferFrançois Légaré
Feb 1, 1986·Optics Letters·I Freund, M Deutsch
Aug 20, 2010·Optics Express·Ivan Amat-RoldanDavid Artigas
Oct 14, 2010·Optics Express·Ivan GusachenkoMarie-Claire Schanne-Klein
Feb 18, 2011·Biomedical Optics Express·Maxime RivardFrançois Légaré
Apr 21, 2011·Biophysical Journal·Ping-Jung SuChen-Yuan Dong
Nov 22, 2011·Journal of the Mechanical Behavior of Biomedical Materials·C P BrownH S Gill
Jan 19, 2012·Biomedical Optics Express·Gaël LatourMarie-Claire Schanne-Klein
Jan 28, 2014·Biomedical Optics Express·Cameron P BrownFrancois Légaré

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Citations

Mar 15, 2018·Scientific Reports·Andrew S QuigleySamuel P Veres
May 4, 2018·Scientific Reports·Rayko I StantchevEuan Hendry
Sep 15, 2020·Biomedical Optics Express·Emily G PendletonLuke J Mortensen
Jul 28, 2016·Optics Express·Stéphane BancelinFrançois Légaré
Mar 16, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Carrie K Hui MingaloneLi Zeng
Mar 16, 2016·Biomedical Optics Express·Stéphane BancelinFrançois Légaré
May 2, 2018·Optics Letters·Stéphane BancelinFrançois Légaré
Oct 16, 2018·Optics Letters·Jarno N van der KolkLora Ramunno
Jun 29, 2021·Frontiers in Bioengineering and Biotechnology·Martin VielreicherOliver Friedrich
Dec 19, 2020·ACS Photonics·Guy MalkinsonWilly Supatto

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