Analysis of full coding sequence of the TP53 gene in invasive vulvar cancers: Implications for therapy

Gynecologic Oncology
Karl Kashofer, Sigrid Regauer

Abstract

This study evaluates the frequency and type of TP53 gene mutations and HPV status in 72 consecutively diagnosed primary invasive vulvar squamous cell carcinomas (SCC) during the past 5years. DNA of formalin-fixed and paraffin embedded tumour tissue was analysed for 32 HPV subtypes and the full coding sequence of the TP53 gene, and correlated with results of p53 immunohistochemistry. 13/72 (18%) cancers were HPV-induced squamous cell carcinomas, of which 1/13 (8%) carcinoma harboured a somatic TP53 mutation. Among the 59/72 (82%) HPV-negative cancers, 59/72 (82%) SCC were HPV-negative with wild-type gene in 14/59 (24%) SCC and somatic TP53 mutations in 45/59 (76%) SCC. 28/45 (62%) SCC carried one (n=20) or two (n=8) missense mutations. 11/45 (24%) carcinomas showed a single disruptive mutation (3× frame shift, 7× stop codon, 1× deletion), 3/45 SCC a splice site mutation. 3/45 (7%) carcinomas had 2 or 3 different mutations. 18 different "hot spot" mutations were observed in 22/45 cancers (49%; 5× R273, 3× R282; 2× each Y220, R278, R248). Immunohistochemical p53 over expression was identified in most SCC with missense mutations, but not in SCC with disruptive TP53 mutations or TP53 wild-type. 14/45 (31%) patients with TP53 mutated...Continue Reading

Citations

Aug 5, 2017·Viruses·Tamara R LitwinNicolas Wentzensen
Jul 25, 2018·Oncology Research and Treatment·Louisa T HamiDirk M Forner
Oct 7, 2018·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Sigrid RegauerOlaf Reich
Aug 15, 2020·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Basile Tessier-CloutierLynn N Hoang
Mar 24, 2020·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Basile Tessier-CloutierLynn N Hoang
Dec 18, 2019·Histopathology·Naveena Singh, C Blake Gilks
Apr 27, 2020·Journal of Cancer Research and Clinical Oncology· Giulia MantovaniGiorgia Garganese
Sep 14, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Linda S NooijTjalling Bosse
Oct 27, 2020·Journal of Lower Genital Tract Disease·Debra S HellerUNKNOWN ISSVD Difficult Pathologic Diagnoses Committee
Oct 10, 2020·Seminars in Diagnostic Pathology·Deyin Xing, Oluwole Fadare
Nov 10, 2020·Gynécologie, Obstétrique, Fertilité & Sénologie·S GuillonR Rouzier

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