May 1, 1985

Analysis of gangliosides using fast atom bombardment mass spectrometry

Chemistry and Physics of Lipids
H EggeG Tettamanti

Abstract

The native gangliosides GM3, GM1, Fuc-GM1, GD1a, GD1b, Fuc-GD1b, GT1b and GQ1b were analysed by fast atom bombardment mass spectrometry (FAB-MS) in the negative ion mode in a matrix of thioglycerol. After permethylation the same gangliosides were analysed by electron impact (EI) and FAB-MS in the positive ion mode. The negative ion mass spectra furnished information on the molecular weight, the ceramide moiety and the sequence of carbohydrate residues. The sites of attachment and the number of sialic acids present could be deduced directly from the pattern of sequence ions. After addition of sodium acetate positive ion FAB-spectra of the permethylated samples show intense pseudomolecular ions M + Na, that provide evidence on the homogeneity of the samples. In addition, the ceramide part, the oligosaccharide moiety obtained after cleavage of the glycosidic bond of the hexosamine residue, the whole carbohydrate chain and the sialic acids are represented by specific fragment ions. With EI-MS further information can be obtained on the sphingosine and fatty acid components of the ceramide residue. The data show, that the combination of soft ionization mass spectrometry with classical EI-MS gives valuable information on the structure...Continue Reading

Mentioned in this Paper

Spectrometry, Mass, Fast Atom Bombardment
Ceramides
Glycolipids
Cytokinesis of the Fertilized Ovum
Gangliosides
Mass Spectrometry
Saturated Fat
Methylation
Brain Chemistry
Cytokinesis

About this Paper

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