PMID: 8596534Feb 15, 1996Paper

Analysis of gene- and strand-specific repair in the moderately UV-sensitive Saccharomyces cerevisiae rad23 mutant

Mutation Research
R VerhageJaap Brouwer

Abstract

The RAD23 gene of Saccharomyces cerevisiae is involved in nucleotide excision repair (NER) and mutations in this gene confer a moderate sensitivity to UV irradiation. However, no repair of either cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts, the major types of lesions formed upon UV irradiation, was detectable during the first 4 h post UV irradiation in a rad23 mutant. rad23, like the rad7 and rad16 mutants, is not as UV sensitive as completely NER-deficient mutants. The rad7 and rad16 mutants are only partly defective in NER: non-transcribed strands are completely refractory to repair while transcription-coupled repair is not affected. To investigate whether the rad23 mutant has similar strand-specific repair characteristics we analyzed gene-specific CPD removal from several loci using strand-specific probes but did not detect any repair. The moderate UV sensitivity of rad23 mutants as compared to completely NER-deficient mutants is therefore not due to gene- or strand-specific removal of lesions, indicating that rad23 mutants do not have a similar repair defect as rad7 or rad16 mutants, but are presumably defective in general NER. The rad23 mutation does not suppress the high UV sensitivity of completely NER-def...Continue Reading

References

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Citations

Feb 1, 2000·Biochemical and Biophysical Research Communications·M LombaertsJ Brouwer
Nov 7, 2002·Current Genetics·Lori LommelKevin S Sweder
Aug 1, 2000·Mutation Research·S J McCreadyA Yasui
Aug 1, 1998·Mutation Research·E J VonarxB A Kunz
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·A Sturm, S Lienhard
Nov 11, 2008·The Journal of Biological Chemistry·Shirong YuSimon H Reed
Jan 26, 2002·Molecular and Cellular Biology·Jessica M Y NgGijsbertus T J van der Horst
Oct 17, 2008·Molekuliarnaia biologiia·J Y Wu, G S Yamg
Nov 18, 2011·Molecular Biology International·Hannes Lans, Wim Vermeulen
Jun 16, 2010·Acta histochemica·Armen MardirosPaula L Fischhaber
Mar 7, 1998·Nature·C SchauberK Madura
Mar 22, 2001·The Journal of Biological Chemistry·T SuzukiW J Lennarz
Dec 5, 1998·The Journal of Biological Chemistry·L E JansenJ Brouwer
Sep 1, 1996·Molecular and Cellular Biology·K SugasawaF Hanaoka

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