Analysis of gene expression changes in A549 cells induced by organic compounds from respirable air particles

Mutation Research
Helena LíbalováJan Topinka

Abstract

A number of toxic effects of respirable ambient air particles (genotoxic effects, inflammation, oxidative damage) have been attributed to organic compounds bound onto the particle surface. In this study, we analyzed global gene expression changes caused by the extractable organic matters (EOMs) from respirable airborne particles <2.5μm (PM2.5), collected at 3 localities from heavily polluted areas of the Czech Republic and a control locality with low pollution levels, in human lung epithelial A549 cells. Although the sampled localities differed in both extent and sources of air pollution, EOMs did not induce substantially different gene expression profiles. The number of transcripts deregulated in A549 cells treated with the lowest EOM concentration (10μg/ml) ranged from 65 to 85 in 4 sampling localities compared to the number of transcripts deregulated after 30μg/ml and 60μg/ml of EOMs, which ranged from 90 to 109, and from 149 to 452, respectively. We found numerous commonly deregulated genes and pathways related to activation of the aryl hydrocarbon receptor (AhR) and metabolism of xenobiotics and endogenous compounds. We further identified deregulation of expression of the genes involved in pro-inflammatory processes, oxida...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jing LiMarc B Hershenson
Oct 16, 2002·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jeanelle M MartinezNigel J Walker
Apr 11, 2003·Biochimica Et Biophysica Acta·S KomatsuN Kato
Dec 25, 2003·Bioinformatics·Jelle J GoemanHans C van Houwelingen
Jan 4, 2005·Biochemical Pharmacology·Alvaro PugaYing Xia
Aug 23, 2005·Cancer Letters·Yuki TsuchiyaTsuyoshi Yokoi
Dec 16, 2005·Environmental and Molecular Mutagenesis·Maria E Gutiérrez-CastilloPatricia Ostrosky-Wegman
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
Nov 28, 2006·Nature Reviews. Cancer·Daniel W Nebert, Timothy P Dalton
Jul 17, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Brigham C Willis, Zea Borok
Mar 25, 2008·Biochemical and Biophysical Research Communications·Fumiaki OhtakeShigeaki Kato
May 10, 2008·Bioinformatics·Pan DuSimon M Lin
May 22, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Fabiola Castorena-TorresArnulfo Albores
Sep 27, 2008·Biochemical Pharmacology·Alvaro PugaJennifer L Marlowe
Sep 24, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Maurizio GualtieriMarina Camatini
Dec 20, 2011·Toxicology Letters·Maurizio GualtieriCristina Battaglia

❮ Previous
Next ❯

Citations

Oct 12, 2019·International Journal of Molecular Sciences·Sara MarchettiParide Mantecca
Sep 15, 2020·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Margaux CochardYann Landkocz
Dec 28, 2019·International Journal of Molecular Sciences·Miguel Santibáñez-AndradeClaudia M García-Cuellar
Dec 19, 2019·International Journal of Molecular Sciences·Pavlína ŠimečkováMiroslav Machala

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis