Analysis of gene expression of bifidobacteria using as the reporter an anaerobic fluorescent protein

Biotechnology Letters
Cynthia Montenegro-RodríguezJosé M Landete

Abstract

To determine the effectiveness of evoglow-Pp1 as a reporter to study gene expression in bifidobacteria. To choose a strong and constitutive promoter to track fluorescently labelled bifidobacteria in environments under anaerobic conditions. The elongation factor P (EF-P) promoter from Bifidobacterium longum CECT 4551 produced the highest emission of fluorescence signal and was therefore able to produce the highest gene expression of the promoters studied. The promoters from B. longum CECT 4551 showed different fluorescence signal intensities which, in descending order, were: EF-P, initiation factor IF-2, elongation factor G, elongation factor Tu, elongation factor Nus A, elongation factor Ts and 30S ribosomal protein S12. The consistency of the methods employed (fluorescence imaging system, fluorescence microscopy, fluorimetry and flow cytometry) showed that the construction pNZ:Prom.GFPana contained the anaerobic fluorescent protein evoglow-Pp1 could be exploited as a tool for analysing the gene expression in bifidobacteria strains.

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Citations

Jun 6, 2016·World Journal of Microbiology & Biotechnology·José M LandeteJuan L Arqués
Aug 8, 2017·International Journal of Molecular Sciences·José María Landete, Juan Luis Arqués
Nov 21, 2020·Applied Microbiology and Biotechnology·Susana LangaJosé María Landete

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