Analysis of gene expression patterns by microarray hybridization in blood mononuclear cells of SLA-DRB1 defined Canadian Yorkshire pigs.

BMC Research Notes
Maria I Nino-SotoBonnie A Mallard

Abstract

The Swine Leukocyte Antigen (SLA) system encodes molecules for self-nonself discrimination and is associated with immune responses and disease resistance. Three lines of pigs defined by their SLA-DRB1 alleles were developed at the University of Guelph for xenotransplantation and immune response studies. The aim of this project was to explore the potential association between defined SLA-DRB1 alleles and gene transcriptional patterns of other immune-related genes in blood mononuclear cells. Three SLA-DRB1 alleles were characterized using a RT-PCR-based sequencing method. The loci represented included a new allele, DRB1*04ns01. Next, microarray heterologous (bovine-porcine) hybridization together with qPCR were used to explore differential gene expression between SLA-DRB1-defined groups. Microarray analysis showed significant (p < 0.01) differential expression for 5 genes, mostly related to inflammation. Genes varied according to the comparison analyzed. Further testing with qPCR revealed the same trend of differential expression for 4 of the genes, although statistical significance was reached for only one. A new SLA-DRB1 allele was characterized. A potential association was found between SLA-DRB1 alleles and inflammation-relate...Continue Reading

References

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Mar 27, 2007·International Journal of Biological Sciences·Joan K Lunney

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Datasets Mentioned

BETA
EU087427
EU087428
GSE7908

Methods Mentioned

BETA
PCR

Software Mentioned

ClustalW
Basic Local Alignment Search Tool
SAS
Acuity
Relative Quantification
MIAME
BLAST

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