Analysis of Gene Expression Signatures in Cancer-Associated Stroma from Canine Mammary Tumours Reveals Molecular Homology to Human Breast Carcinomas

International Journal of Molecular Sciences
Julia EttlinEnni Markkanen

Abstract

Cancer-associated stroma (CAS) plays a key role in cancer initiation and progression. Spontaneously occurring canine mammary carcinomas are viewed as excellent models of human breast carcinomas. Considering the importance of CAS for human cancer, it likely plays a central role in canine tumours as well. So far, however, canine CAS lacks characterisation, and it remains unclear whether the biology between CAS from canine and human tumours is comparable. In this proof-of-principle study, using laser-capture microdissection, we isolated CAS and normal stroma from 13 formalin-fixed paraffin embedded canine simple mammary carcinomas and analysed the expression of seven known human CAS markers by RT-qPCR (Reverse Transcription quantitative PCR) and validated some targets by immunohistochemistry. We found that Col1a1 (Collagen1α1), αSMA (alpha Smooth Muscle Actin), FAP (Fibroblast activation protein), PDGFRβ (Platelet-derived growth factor receptor beta), and Caveolin-1 were significantly upregulated in canine CAS, and the expression of CXCL12 (Stromal cell derived factor 1) significantly decreased, whereas MMP2 (Matrix Metalloproteinase 1) and IL6 (Interleukin 6) did not change. Our results suggest strong similarities in CAS biology ...Continue Reading

References

May 15, 1988·International Journal of Cancer. Journal International Du Cancer·A P SappinoG Gabbiani
Mar 10, 2001·Experimental Cell Research·B Elenbaas, R A Weinberg
Mar 20, 2002·Nature Reviews. Cancer·M J Bissell, D Radisky
Jan 21, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Vladimir VincekAzorides R Morales
Jul 21, 2004·Cancer Cell·Minna AllinenKornelia Polyak
Jan 4, 2005·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Cui YazhouWu Licun
May 5, 2005·PLoS Biology·Robert B WestMatt van de Rijn
Mar 31, 2006·Nature Reviews. Cancer·Raghu Kalluri, Michael Zeisberg
Oct 24, 2006·Breast Cancer Research : BCR·Greg FinakMorag Park
Jan 31, 2007·Breast Cancer Research : BCR·Carine ChaveyGwendal Lazennec
Apr 5, 2007·Nature Protocols·Virginia EspinaLance A Liotta
Nov 30, 2007·The Journal of Pathology·A BergamaschiA-L Børresen-Dale
Dec 7, 2007·The Journal of Clinical Investigation·Zachary T Schafer, Joan S Brugge
Apr 17, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Andrew H BeckRobert B West
Apr 29, 2008·Nature Medicine·Greg FinakMorag Park
Aug 21, 2008·Nature Reviews. Genetics·Elinor K Karlsson, Kerstin Lindblad-Toh
Sep 4, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jozien HellemanEls M J J Berns
Feb 4, 2009·Breast Cancer Research : BCR·Xiao-Jun MaDennis C Sgroi
May 5, 2009·The American Journal of Pathology·Agnieszka K WitkiewiczMichael P Lisanti
Oct 28, 2010·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Masako YamashitaTaizo Shiraishi
Nov 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yasushi KojimaAkira Orimo
Jan 29, 2011·The Veterinary Journal·Andreia SantosAugusto de Matos
Mar 4, 2011·Methods in Molecular Biology·Renate Burgemeister
Mar 29, 2011·Trends in Molecular Medicine·Jennie L RowellCarlos E Alvarez
Jul 6, 2011·BMC Veterinary Research·Luca AresuMauro Dacasto
Mar 24, 2012·Cancer Cell·Douglas Hanahan, Lisa M Coussens
Oct 16, 2012·Pharmacology & Therapeutics·Jing Zhang, Jinsong Liu
Apr 16, 2013·The American Journal of Pathology·Oliver FringsErik L L Sonnhammer
Apr 17, 2014·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Matthew L MortonCheryl L Thompson
Apr 25, 2014·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Hitomi ShinodaWilliam H Hanneman
Aug 26, 2014·Future Oncology·Janna PaulssonArne Östman
Feb 24, 2015·Nature Genetics·Alexandre CalonEduard Batlle
Mar 25, 2015·Nature Reviews. Cancer·Ubaldo E Martinez-OutschoornMichael P Lisanti

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Citations

Apr 1, 2020·International Journal of Molecular Sciences·Joanna K BujakKinga Majchrzak-Kuligowska
Oct 14, 2019·Veterinary and Comparative Oncology·Joana ReisPedro Faísca
Jan 11, 2020·Frontiers in Cell and Developmental Biology·Enni Markkanen
Jul 28, 2018·Cancer Immunology, Immunotherapy : CII·Raj K KurupatiHildegund C J Ertl
Dec 4, 2019·Frontiers in Oncology·Kendall L LangstenJaime F Modiano
Sep 4, 2020·Diagnostics·Anca OnaciuIoana Berindan-Neagoe
Dec 8, 2020·Frontiers in Oncology·Asadoor Amirkhani NamagerdiAntonio Giordano

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Methods Mentioned

BETA
xenograft
laser-capture microdissection
Laser Capture Microdissection
Biopsy
PCR

Software Mentioned

GraphPad Prism
4200
2200 Tape Station

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