Analysis of genes, transcripts, and proteins via DNA ligation

Annual Review of Analytical Chemistry
Tim ConzeU Landegren

Abstract

Analytical reactions in which short DNA strands are used in combination with DNA ligases have proven useful for measuring, decoding, and locating most classes of macromolecules. Given the need to accumulate large amounts of precise molecular information from biological systems in research and in diagnostics, ligation reactions will continue to offer valuable strategies for advanced analytical reactions. Here, we provide a basis for further development of methods by reviewing the history of analytical ligation reactions, discussing the properties of ligation reactions that render them suitable for engineering novel assays, describing a wide range of successful ligase-based assays, and briefly considering future directions.

References

May 25, 1975·Journal of Molecular Biology·F Sanger, A R Coulson
Aug 1, 1991·PCR Methods and Applications·F Barany
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·F Barany
Aug 26, 1988·Science·U LandegrenL Hood
Nov 29, 1974·Science·I R Lehman
Sep 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·K KleppeH G Khorana
Aug 23, 1967·Biochemical and Biophysical Research Communications·N R CozzarelliA Kornberg
Feb 25, 1995·Nucleic Acids Research·K AbravayaH H Lee
Jun 25, 1994·Nucleic Acids Research·S M GryaznovR L Letsinger
Aug 1, 1996·Nucleic Acids Research·J LuoF Barany
Sep 1, 1997·Nucleic Acids Research·C E Pritchard, E M Southern
Feb 28, 1998·Nucleic Acids Research·V Sriskanda, S Shuman
Jun 17, 1998·Nucleic Acids Research·J Liu, J S Taylor
Jul 22, 1998·Nucleic Acids Research·V Sriskanda, S Shuman
Nov 4, 1998·Nucleic Acids Research·J BanérU Landegren
Jan 8, 1999·Journal of Molecular Biology·A J Doherty, D B Wigley
Jan 16, 1999·Nucleic Acids Research·Y Xu, E T Kool
May 13, 1999·The Journal of Biological Chemistry·M Odell, S Shuman
Sep 24, 1999·Journal of Molecular Biology·N P GerryF Barany
Sep 25, 1999·Nucleic Acids Research·A S BhagwatT Lindahl
Feb 5, 2000·Journal of Molecular Biology·A J Doherty, T R Dafforn
Jun 28, 2000·Nucleic Acids Research·D O AntsonM Nilsson
Jul 11, 2000·Nature Biotechnology·M NilssonU Landegren
Jul 11, 2000·Nature Genetics·E Dequeker, J J Cassiman
Jan 5, 2001·Nucleic Acids Research·M NilssonU Landegren

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Citations

Aug 24, 2010·Analytical and Bioanalytical Chemistry·Reham Helwa, Jörg D Hoheisel
Nov 10, 2013·Nucleic Acids Research·Gregory J S LohmanThomas C Evans
Sep 13, 2012·Sensors·Chia-Chen ChangChii-Wann Lin
Apr 22, 2014·Trends in Immunology·Rahul Satija, Alex K Shalek
May 18, 2010·Biochemical and Biophysical Research Communications·Yingfeng AnWenfang Wu
Aug 5, 2015·Nucleic Acids Research·Marco MignardiMats Nilsson
Apr 2, 2016·Analytical and Bioanalytical Chemistry·Cheulhee Jung, Andrew D Ellington
Sep 19, 2014·PloS One·Junhong YanMasood Kamali-Moghaddam
Sep 17, 2013·Angewandte Chemie·Hongquan ZhangX Chris Le
Oct 15, 2013·Electrophoresis·Gi Won ShinGyoo Yeol Jung
Sep 20, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Magnus StougaardBirgitta R Knudsen
May 28, 2019·Current Molecular Medicine·Somayeh Heidari SharafdarkolaeeFatemeh Heidari Sharafdarkolaee
Feb 4, 2011·Chemical Society Reviews·Nan Dai, Eric T Kool
Jul 1, 2020·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Cecilia LindskogFredrik Pontén
Oct 28, 2010·Analytical Chemistry·Jenny GöranssonMats Nilsson
Dec 13, 2012·Chemical Reviews·Hongquan ZhangX Chris Le

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Methods Mentioned

BETA
genotyping
oligonucleotide
dot-blot
for
PCR
electrophoresis
immunoprecipitation
Assay
proximity ligation assay
proximity ligation

Software Mentioned

SOLiD
3PLA
PLA

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