Analysis of genetic polymorphisms and tropism in East African Leishmania donovani by Amplified Fragment Length Polymorphism and kDNA minicircle sequencing

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Hanan T JaberCharles L Jaffe

Abstract

Visceral leishmaniasis (VL), the most severe form of leishmaniasis, is caused by Leishmania donovani. In addition to fatal VL, these parasites also cause skin diseases in immune-competent and -suppressed people, post-kala azar dermal leishmaniasis (PKDL) and HIV/VL co-infections, respectively. Genetic polymorphism in 36 Ethiopian and Sudanese L. donovani strains from VL, PKDL and HIV/VL patients was examined using Amplified Fragment Length Polymorphism (AFLP), kDNA minicircle sequencing and Southern blotting. Strains were isolated from different patient tissues: in VL from lymph node, spleen or bone marrow; and in HIV/VL from skin, spleen or bone marrow. When VL and PKDL strains from the same region in Sudan were examined by Southern blotting using a DNA probe to the L. donovani 28S rRNA gene only minor differences were observed. kDNA sequence analysis distributed the strains in no particular order among four clusters (A - D), while AFLP analysis grouped the strains according to geographical origin into two major clades, Southern Ethiopia (SE) and Sudan/Northern Ethiopia (SD/NE). Strains in the latter clade were further divided into subpopulations by zymodeme, geography and year of isolation, but not by clinical symptoms. Howev...Continue Reading

Citations

Jul 3, 2020·PLoS Neglected Tropical Diseases·Mallikarjuna Rao GeddaShyam Sundar
Aug 21, 2021·Comparative Immunology, Microbiology and Infectious Diseases·Mohammed Hassan FlaihKhwam Reissan Hussein

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Datasets Mentioned

BETA
KY950645
KY950679

Methods Mentioned

BETA
GTPase
PCR
electrophoresis
genotyping
PCRs
biopsy
the

Software Mentioned

Peak Scanner
STRUCTURE
net
Neighbor
Structure harvester
Splitstree
Muscle
tinyCAT
MultAlin
BioNJ

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