PMID: 7529554Oct 1, 1994Paper

Analysis of glioma cell lines for amplification and overexpression of MDM2

Genes, Chromosomes & Cancer
J HeC D James

Abstract

Recently, amplification of the gene encoding a p53 binding protein, MDM2, was determined in 8% of the cases constituting a large series of glioblastomas. Here we have utilized Southern blot analysis to examine 30 cell lines established from such tumors, and our investigation has revealed large increases in MDM2 gene dosage in two cases, one of which showed coamplification of the CDK4 gene that resides in close proximity to MDM2 in chromosomal region 12q13-14. Northern analysis demonstrated overexpression of MDM2 mRNA in the two cell lines with gene amplification, and overexpression of MDM2 protein was evident in each of these by immunohistochemical and Western blot analysis. Analysis of TP53 cDNAs revealed normal TP53 sequences in the cell lines with MDM2 amplification; these results are consistent with those of previous studies suggesting that MDM2 amplification occurs only in tumors expressing wild-type p53. In total, these data suggest that MDM2 amplification in glioblastoma cell lines occurs at a frequency (6.7%) comparable to that determined in primary tumors; occurs in cell lines expressing wild-type p53; and can involve the coamplification of additional genes.

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Citations

Apr 17, 2008·Brain Tumor Pathology·Yoshiyuki TanakaToru Itakura
Sep 29, 2006·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Cristóbal Belda-IniestaManuel González Barón
Jul 15, 2009·Nature Reviews. Neurology·Benjamin Purow, David Schiff
Mar 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sung-Suk SuhCarlo M Croce
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Aug 27, 2013·PloS One·Barbara CostaClaudia Martini
Nov 4, 2008·Journal of Neurosurgery·Rina G KhatriRobert J Weil
Sep 10, 2009·Annual Review of Pathology·Candece L GladsonWei Michael Liu
Nov 23, 2006·Hematology/oncology Clinics of North America·Yelena Lyustikman, Andrew B Lassman
Oct 22, 2004·Seminars in Oncology·Ravi D Rao, C David James
Nov 7, 2003·Genes, Chromosomes & Cancer·Ajay PanditaC David James
Mar 17, 2001·Nature Reviews. Genetics·E C Holland
Dec 18, 2014·World Journal of Gastroenterology : WJG·Yu-Tien ChangChing-Huang Lai
May 20, 2016·Cold Spring Harbor Perspectives in Medicine·Jonathan D OlinerSean Caenepeel
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Oct 3, 2020·Cancer Cell International·Wangsheng ChenJianhua Zhang
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