Analysis of glucuronide and sulfate steroids in urine by ultra-high-performance supercritical-fluid chromatography hyphenated tandem mass spectrometry

Analytical and Bioanalytical Chemistry
Mickael DouéBruno Le Bizec

Abstract

Profiling conjugated urinary steroids to detect anabolic-steroid misuse is recognized as an efficient analytical strategy in both chemical-food-safety and anti-doping fields. The relevance and robustness of such profiling rely on the analysis of glucuronide and sulfate steroids, which is expected to have properties including accuracy, specificity, sensitivity, and, if possible, rapidity. In this context, the ability of ultra-high-performance supercritical-fluid chromatography (UHPSFC) hyphenated tandem mass spectrometry (MS-MS) to provide reliable and accurate phase II analysis of steroids was assessed. Four stationary phases with sub-2 μm particles (BEH, BEH 2-ethyl-pyridine, HSS C18 SB, and CSH fluorophenyl) were screened for their capacity to separate several conjugated steroid isomers. Analytical conditions including stationary phase, modifier composition and percentage, back pressure, column temperature, and composition and flow rate of make-up solvent were investigated to improve the separation and/or the sensitivity. Thus, an analytical procedure enabling the analysis of eight glucuronide and 12 sulfate steroids by two different methods in 12 and 15 min, respectively, was optimized. The two procedures were evaluated, and...Continue Reading

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Citations

Jan 29, 2016·Journal of Chromatography. a·Stéphanie RomandDavy Guillarme
Aug 25, 2016·Analytical and Bioanalytical Chemistry·Maria Kristina ParrFrancesco Botrè
May 17, 2018·Journal of Molecular Endocrinology·Paul A Foster, Jonathan Wolf Mueller
Dec 19, 2018·Journal of Analytical Toxicology·Fernando X MoreiraPaula G de Pinho
Jan 14, 2021·Drug Testing and Analysis·Fernando MoreiraMaria de Lourdes Bastos
Apr 18, 2021·Drug Testing and Analysis·Jenny SchulzeLena Ekström
May 1, 2021·Biomolecules·Michal KaletaMiroslav Strnad
Jun 22, 2021·Critical Reviews in Analytical Chemistry·Yang Song, Xue-Song Feng
Mar 20, 2021·The Journal of Clinical Endocrinology and Metabolism·Jonathan Wolf MuellerMatthias Kroiss

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