Analysis of glutaminase activity and RNA expression in preimplantation mouse embryos

Molecular Reproduction and Development
C L ChatotC A Ziomek

Abstract

Glutamine is utilized as an energy substrate in preimplantation mouse embryos. Glutaminase is the enzyme responsible for the conversion of glutamine to glutamic acid, which then enters the trichloro acetic acid (TCA) cycle as alpha-ketoglutarate. Glutaminase enzyme activity was assessed in preimplantation embryos that developed in vivo, and glutaminase RNA expression was examined in embryos that developed in vivo or were cultured in CZB medium to various preimplantation stages between 1-cell and blastocyst. Glutaminase activity in 1-8-cell-stage mouse embryos that developed in vivo ranged from 0.009-0.01 U/mg protein (2.39-2.95 x 10(-7) U per embryo) and increased 3-4 fold to 0.034 U/mg protein (8.13 x 10(-7) U per embryo) at the blastocyst stage. Relative stage-specific expression of glutaminase RNA was assessed by reverse transcription polymerase chain reaction (RT-PCR) in embryos that developed both in vivo and in CZB culture. In vivo, glutaminase RNA was expressed at the 1-cell stage, declined to 23% of 1-cell levels at the early 2-cell stage, and reaccumulated from late 2-cell through blastocyst stage, where it reached a high of 204% of 1-cell levels. CZB-cultured embryos exhibited a similar pattern of developmental RNA ex...Continue Reading

References

Oct 1, 1978·The Journal of Experimental Zoology·P Quinn, G M Harlow
Mar 1, 1992·Molecular Reproduction and Development·J A Lawitts, J D Biggers
May 1, 1992·Journal of Reproduction and Fertility·A M Lewis, P L Kaye
Mar 1, 1991·Journal of Reproduction and Fertility·J A Lawitts, J D Biggers
Mar 1, 1990·Biology of Reproduction·C L ChatotC A Ziomek
May 1, 1990·Journal of Reproduction and Fertility·C L ChatotC A Ziomek
Sep 1, 1990·Molecular Reproduction and Development·Z Werb
Jul 1, 1989·Journal of Reproduction and Fertility·C L ChatotI Torres
Dec 1, 1988·Biology of Reproduction·S A Schini, B D Bavister
Jun 1, 1988·Brain Research·C BannerN P Curthoys
Jul 1, 1988·Journal of Reproduction and Fertility·D Rieger, P Guay
Mar 1, 1986·Journal of Reproduction and Fertility·V E Papaioannou, K M Ebert
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R A GravesG A Schultz
Feb 1, 1985·Developmental Biology·D H GiebelhausG A Schultz
Jan 1, 1985·Methods in Enzymology·E KvammeG Svenneby
Mar 15, 1973·Experimental Cell Research·P C Cross, R L Brinster
Jan 1, 1973·Experimental Cell Research·R B Gwatkin, A A Haidri
Aug 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J D BiggersR P Donahue
Nov 1, 1968·Journal of Reproduction and Fertility·W K Whitten, J D Biggers
Oct 1, 1965·Journal of Reproduction and Fertility·R L Brinster
Jun 1, 1967·Journal of Reproduction and Fertility·R L Brinster
May 1, 1994·Biology of Reproduction·G T ErbachJ D Biggers
Apr 1, 1993·Molecular Reproduction and Development·J D BiggersC P Lechene

❮ Previous
Next ❯

Citations

Jun 17, 2009·Seminars in Cell & Developmental Biology·R DumollardK Swann
Jul 20, 2017·Molecular Reproduction and Development·Kailun Hu, Yang Yu
Feb 6, 2020·International Journal of Molecular Sciences·Maria SchindlerAnne Navarrete Santos
Sep 5, 2006·Human Reproduction·Marcin JózwikFrederick C Battaglia

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.