Analysis of glycoproteins, glycopeptides and glycoprotein-derived oligosaccharides by high-performance capillary electrophoresis

Journal of Chromatography. a
K Kakehi, S Honda

Abstract

Recent developments in the analysis of glycoproteins by high-performance capillary electrophoresis are reviewed, with emphasis on their carbohydrate chains. Glycoforms of glycoproteins were directly separated from each other by careful optimization of the analytical conditions. Glycopeptides in tyrptic digests were separated and the peptides carrying glycosylation sites were differentiated from others. Released oligosaccharide chains were separated from each other by direct or modified zone electrophoresis and directly detected by measuring the UV absorption at a low wavelength. Precolumn derivatization by various methods extended the utility of both the separation mode and detection technique. Dual mode analysis after derivatization permitted reliable identification and quantification without references.

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Citations

Dec 28, 1999·Biomedical Chromatography : BMC·N K Karamanos, F Lamari
Feb 24, 2001·Electrophoresis·J C WatervalW J Underberg
Feb 10, 1998·Electrophoresis·H A BardelmeijerW J Underberg
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Jan 29, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kae SatoSunao Yamazaki
May 24, 2014·Mass Spectrometry Reviews·Anthonius A M HeemskerkOleg A Mayboroda
Mar 3, 2005·Journal of Pharmaceutical and Biomedical Analysis·Yu-ki MatsunoKazuaki Kakehi
Aug 19, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Maria BergströmSten Ohlson
Feb 24, 2009·Proteomics·Katrin SparbierMarkus Kostrzewa
Dec 16, 1998·Electrophoresis·S Suzuki, S Honda
Dec 16, 1998·Electrophoresis·F T Chen, R A Evangelista
Jul 10, 1997·Nature·P M RuddR A Dwek
Dec 18, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shigeo Suzuki

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