PMID: 9092388Jan 1, 1995Paper

Analysis of glycosylated A1c hemoglobin by liquid phase chromatography and immunoagglutination

Archives de l'Institut Pasteur de Tunis
M SellamiS Fattoum


Glycated hemoglobin A1c assay is performed using HPLC on cation exchange TSK SP 5 PW column. Separation is obtained in 10 minutes using graduated elution with sodium chloride and Bis-Tris buffer. Hb A1c is resolved from the other hemoglobins (F, A0 and S). Reproductibility of method is quite good and correlated with an immunologic method (DCA 2000).

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