PMID: 9092388Jan 1, 1995Paper

Analysis of glycosylated A1c hemoglobin by liquid phase chromatography and immunoagglutination

Archives de l'Institut Pasteur de Tunis
M SellamiS Fattoum

Abstract

Glycated hemoglobin A1c assay is performed using HPLC on cation exchange TSK SP 5 PW column. Separation is obtained in 10 minutes using graduated elution with sodium chloride and Bis-Tris buffer. Hb A1c is resolved from the other hemoglobins (F, A0 and S). Reproductibility of method is quite good and correlated with an immunologic method (DCA 2000).

Related Concepts

Agglutination Tests
High Pressure Liquid Chromatography Procedure
Ion-Exchange Chromatography Procedure
Hb A1a-2
Hemoglobinopathies
Nested Case-Control Studies

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