PMID: 7296806Jul 1, 1981Paper

Analysis of green coffee bean and castor bean allergens using RAST inhibition

Clinical Allergy
S B LehrerJ E Salvaggio


Coffee workers with occupational allergic symptoms and positive skin tests to green coffee bean and factor dust antigens have elevated serum IgE antibodies (by radioallergosorbent test--RAST) to green coffee and castor bean allergens. These antibodies were used in a RAST inhibition assay to analyse coffee and castor allergens. Bean allergens were extracted by homogenization in PBS, centrifugation and concentration of supernates by ultrafiltration. Green coffee bean allergens, fractionated by gel filtration and Pevikon block electrophoresis, were shown to be very heterogeneous with a molecular weight range of 50 000 to 500 000 daltons. Castor allergens were more homogeneous with a molecular weight of 14 000 daltons and were partially purified by Pevikon block electrophoresis, gel filtration and isoelectrofocusing. Chemical analysis showed that protein was the major component in both allergen extracts. However, proteolytic enzymes could only partially destroy allergenic activity. Such isolation and characterization of these allergens should result in better methods of diagnosis and treatment of coffee workers with occupational allergic disease.


Sep 1, 1978·The Journal of Allergy and Clinical Immunology·R M KarrJ E Salvaggio
Mar 5, 1973·JAMA : the Journal of the American Medical Association·H S Bernton
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Jan 1, 1960·Scandinavian Journal of Clinical and Laboratory Investigation·H J MULLER-EBERHARD
Oct 21, 1961·Nature·S O FREEDMANA H SEHON
Apr 1, 1963·Canadian Journal of Biochemistry and Physiology·A I SIDDIQI, S O FREEDMAN
Mar 1, 1964·The Journal of Allergy·S O FREEDMAN
Mar 1, 1964·The Journal of Allergy·S O FREEDMAN
Nov 1, 1950·The Journal of Allergy·K D FIGLEY, F F A RAWLING
Sep 20, 1938·The Journal of General Physiology·M L Anson

❮ Previous
Next ❯


Jan 1, 1984·Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung·R F Smith
Jul 1, 1985·Clinical Reviews in Allergy·D D Metcalfe
Jun 1, 1985·British Journal of Industrial Medicine·E ZuskinD Butković
Apr 8, 2009·Chest·Marcus OldenburgXaver Baur
Jun 23, 2012·International Archives of Allergy and Immunology·Nikolay ManavskiCordula Bittner

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.