Analysis of haemorrhagic septicaemia-causing isolates of Pasteurella multocida by ribotyping and field alternation gel electrophoresis (FAGE)

Veterinary Microbiology
K M TownsendJ M Papadimitriou

Abstract

Ribotyping and field alternation gel electrophoresis (FAGE) were used to examine 19 Pasteurella multocida isolates, and to assess the ability of these techniques to differentiate P. multocida strains that cause haemorrhagic septicaemia (HS). Reproducible patterns were obtained from both methods, with FAGE demonstrating greater discriminatory power than ribotyping. FAGE analysis was particularly useful in distinguishing North American cultures originating from the 1922 Yellowstone National Park Buffalo 'B' strain, demonstrating the ability to detect genetic alterations induced by repeated subculture. A remarkable homogeneity was observed among Asian HS strains following ribotyping and FAGE analysis, with a clear distinction observed between virulent and avirulent HS isolates. This study has illustrated the value of genomic fingerprinting methods in distinguishing strains of similar serotype, and the capability of these methods to produce detailed characterisation of P. multocida isolates.

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Citations

Dec 2, 2006·Veterinary Research Communications·M K SaxenaB Sharma
Jul 9, 2013·Biologicals : Journal of the International Association of Biological Standardization·Javid ArifPervez Akhtar
Dec 16, 1998·Veterinary Microbiology·K M TownsendA J Frost
Mar 4, 2000·Veterinary Microbiology·M L HuntK M Townsend
Jun 17, 2011·Animal Health Research Reviews·S B ShivachandraA A Kumar
Jan 16, 1998·Research in Veterinary Science·K M TownsendJ M Papadimitriou
Aug 7, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yanhong WangXiufan Liu
Mar 17, 2007·Scandinavian Journal of Infectious Diseases·Ko ChangChun-Lu Lin
Nov 13, 1998·Journal of Bacteriology·M L HuntB Adler
May 23, 1998·Journal of Clinical Microbiology·K M TownsendH J Dawkins

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