Analysis of heart-infiltrating T-cell clonotypes in experimental autoimmune myocarditis in rats

Circulation Research
H HanawaA Shibata

Abstract

Experimental autoimmune myocarditis (EAM) resembles the lethal giant cell myocarditis seen in humans, and the recurrent forms lead to dilated cardiomyopathy (DCM). EAM in rats induced by a subcutaneous injection of cardiac myosin has been shown to be a T cell-mediated autoimmune disease. Alpha beta T cells have proved to be important by the observation that antibodies to alpha beta T-cell receptor (TCR) prevent disease progression. Alpha beta cells recognize antigenic peptides bound to major histocompatibility (MHC) molecules by alpha beta TCR, and complementarity determining region 3 (CDR3) is considered the most important region for antigen recognition. To elucidate the nature of this T cell-mediated myocarditis, we analyzed TCR V beta chains of heart-infiltrating T cells. In the early state of EAM, none of 22 TCR V beta chain transcripts seemed to be dominant by reverse transcription-polymerase chain reaction analysis of total RNA and flow cytometric analysis. On the other hand, single-strand conformation polymorphism analysis of TCR V beta 8.2, V beta 8.5, V beta 10, and V beta 16 cDNA amplified by polymerase chain reaction encompassing the CDR3 revealed oligoclonal expansion in heart-infiltrating T cells isolated from anim...Continue Reading

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Citations

Mar 14, 2001·European Journal of Pharmacology·Z P ZongM L Fu
Jul 20, 2011·BMC Immunology·Chandirasegaran MassilamanyJay Reddy
Feb 5, 2014·Heart and Vessels·Chiharu KishimotoKagemasa Kuribayashi
Dec 6, 2011·Cardiovascular Therapeutics·Jun-ichi SuzukiMitsuaki Isobe
Apr 20, 2007·International Journal of Cardiology·Zhi-Ping ZongMichael Lx Fu
Jun 29, 2005·International Journal of Cardiology·Michelle M KittlesonJoshua M Hare
Feb 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y MatsumotoM Sugisaki

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