Analysis of Helios gene expression and Foxp3 TSDR methylation in the newly diagnosed Rheumatoid Arthritis patients

Immunological Investigations
Parisa ZafariMahdi Taghadosi

Abstract

The control of auto-reactive cells is defective in rheumatoid arthritis (RA). Regulatory T (Treg) cells which play a key role in the modulation of immune responses have an impaired function in RA. Foxp3 is a master regulator of Treg cells which its expression is under the tight control of epigenetic mechanisms. In the current study, we analyzed the epigenetic modulation of the Foxp3 Treg-specific demethylated region (TSDR) and Helios gene expression to determine Treg cells alteration in RA patients. We have recruited 20 newly diagnosed patients with RA and 41 healthy controls in our study. The measurement of Foxp3 and Helios gene expression was performed by the real-time PCR technique and the methylation level of TSDR was analyzed by bisulfite treatment and quantitative methylation-specific PCR (Q-MSP). We found that RA patients had significantly lower level of Foxp3 gene expression and TSDR demethylation compared to healthy subjects (P < 0.001 and P = 0.006, respectively). Inversely, the Helios gene expression was elevated significantly in RA patients group (P = 0.048). We also observed a significant correlation between Foxp3 and Helios gene expression (P = 0.016) as well as a significant correlation between FoxP3 expression a...Continue Reading

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Citations

Mar 21, 2020·Frontiers in Immunology·Yi-Shu HuangTrevor W Stone
Nov 12, 2019·Frontiers in Cellular and Infection Microbiology·Yang SunLianbo Xiao
Dec 29, 2020·Frontiers in Immunology·Agnieszka Paradowska-GoryckaAndrzej Pawlik
Apr 20, 2021·Frontiers in Immunology·Dorota Iwaszkiewicz-GrzesPiotr Trzonkowski
Jul 15, 2021·Disease Markers·Wen-Qing YuMing-Shun Zhang
Jul 21, 2021·Inflammopharmacology·Ehsan NourbakhshFarnoosh Ebrahimzadeh

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