Analysis of hepatitis C virus core protein interaction domains

The Journal of General Virology
O NolandtH G Kräusslich

Abstract

Hepatitis C virus (HCV) core protein forms the internal viral coat that encapsidates the genomic RNA and is enveloped in a host cell-derived lipid membrane. As the single capsid protein, core should be capable of multimerization but attempts to produce virus-like particles following expression of HCV structural proteins have not been successful. In this study, we have analysed the interaction capacity of full-length and truncated HCV core using the yeast two-hybrid system. Full-length core containing or lacking the translocation signal for the E1 glycoprotein did not interact with full-length or truncated core proteins. Truncation to the N-terminal 122 aa revealed an interaction domain which was mapped to the tryptophan-rich sequence from aa 82-102 and was termed the main homotypic interaction domain. The C-terminal hydrophobic fragment of core (aa 122-172) was incapable of interacting with itself but interacted with the main homotypic interaction domain in trans (the weak heterotypic interaction domain). Core proteins truncated at their N and C termini (aa 46-102) were trans-activating when fused to the DNA-binding domain of GAL4. Based on our results, we suggest that the C-terminal segment may interact in cis with the main ho...Continue Reading

Citations

Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A SabileG Barba
Sep 26, 2013·Drug Discovery Today. Technologies
Sep 11, 2007·Advanced Drug Delivery Reviews·Tetsuro SuzukiTakaji Wakita
Feb 22, 2014·Immunologic Research·Katarzyna Gawlik, Philippe A Gallay
Feb 22, 2005·Biochemical and Biophysical Research Communications·Raghavan Bhuvanakantham, Mah-Lee Ng
Oct 1, 2010·The Journal of General Virology·G MousseauA D Strosberg
Feb 5, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Saba KhaliqAsim Pervaiz
Aug 1, 2010·Viruses·Arthur Donny StrosbergGuillaume Mousseau
Oct 19, 2006·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·M Irshad, Indranil Dhar
Aug 3, 2007·Journal of Gastroenterology·Tetsuro SuzukiTakaji Wakita
Mar 12, 2005·The Journal of Biological Chemistry·Sébastien BoniAnnie Cahour
Jan 9, 2008·Journal of Viral Hepatitis·A J Pérez-BernáJ Villalaín
Jun 8, 2007·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Xiaoyan FengShigan Ling
Mar 29, 2012·Canadian Journal of Microbiology·Jean-Baptiste DuvignaudDenis Leclerc
Jul 23, 2004·The Journal of General Virology·Shao-Hung WangShiau-Ting Hu
Dec 28, 2002·Journal of Virology·Kumiko YoshimatsuJiro Arikawa
Aug 17, 2005·Journal of Virology·Steeve BoulantJean-Pierre Lavergne
Sep 6, 2005·Journal of Virology·Nicolas Le MayMichèle Bouloy
Jun 22, 2000·The Journal of General Virology·R Bartenschlager, V Lohmann
Oct 19, 2001·The Journal of Biological Chemistry·W LuJ H Ou Jh
Aug 3, 2001·Journal of Virology·P MaillardA Budkowska
Sep 8, 2012·Journal of Structural and Functional Genomics·Alexander Zawaira, Youtaro Shibayama
Sep 24, 1999·Biochemical and Biophysical Research Communications·T HeintgesJ R Wands

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.