PMID: 7525693Jul 1, 1994Paper

Analysis of hepatitis C virus genotypes by a line probe assay and correlation with antibody profiles

Journal of Hepatology
L J van DoornW Quint

Abstract

The 5' untranslated regions derived from 54 patients with a chronic hepatitis C virus infection were analyzed to determine the (sub)type of hepatitis C virus. Labelled polymerase chain reaction products from 5' untranslated region were used as probes for reverse hybridization in a line probe assay (Inno-LiPA) and results were validated by comparison with direct sequencing data. Five different genotypes could be distinguished based on 5' untranslated region sequence diversity. Results of typing by line probe assay and direct sequencing were similar. Antibody responses against core, NS-3, NS-4 and NS-5 epitopes were detected by RIBA-4 and Inno-LIA HCVAb II confirmatory assays. There was no consistent correlation between genotype and anti-HCV responses, although types 2, 3 and 4 hepatitis C virus isolates show poor reactivity with NS-4 ep!%"pes.

References

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Citations

May 1, 1997·Journal of Medical Virology·R U KhanC A Hart
Jan 13, 2000·Biochemical and Biophysical Research Communications·J S ParkM K Min
Apr 1, 1996·Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme·N Kato, K Shimotohno
May 17, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Kathryn J RolfeMartin D Curran
Mar 28, 1996·The New England Journal of Medicine·E J GaneR Williams
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·L StuyverG Maertens
Apr 24, 2007·Journal of Clinical Gastroenterology·Flor H Pujol, Carmen L Loureiro
Feb 1, 1997·Journal of Gastroenterology and Hepatology·J ChenG W McCaughan
Sep 1, 1995·Journal of Clinical Pathology·J NiuH C Thomas
Feb 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H H FeuchtS Polywka
Apr 19, 2007·Journal of Viral Hepatitis·J TimmT M Allen
Sep 28, 2010·Journal of Medical Virology·Mette Brandt EriksenUNKNOWN DANHEP Group
May 6, 2008·Diagnostic Microbiology and Infectious Disease·Vikas VermaPremashis Kar
Nov 15, 2002·Journal of Viral Hepatitis·P HalfonD Ouzan
Dec 1, 1996·Digestive Diseases and Sciences·C Bréchot
Jan 10, 1998·Clinical and Diagnostic Laboratory Immunology·M DevesaF H Pujol
Apr 1, 1996·Journal of Clinical Microbiology·J DetmerR Sánchez-Pescador
May 1, 1995·Journal of Clinical Microbiology·J M PawlotskyD Dhumeaux
Mar 20, 1998·Annals of Emergency Medicine·T A Morton, G D Kelen
Nov 1, 1996·Journal of Clinical Microbiology·A RavaggiE Cariani

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