Analysis of Histone Modifications in Acute Myeloid Leukaemia Using Chromatin Immunoprecipitation

Methods in Molecular Biology
Benjamin J ShieldsMatthew P McCormack

Abstract

Chromatin Immunoprecipitation (ChIP) using antibodies specific for histone modifications is a powerful technique for assessing the epigenetic states of cell populations by either quantitative PCR (ChIP-PCR) or next generation sequencing analysis (ChIP-Seq). Here we describe the procedure for ChIP of histone marks in myeloid leukaemia cell lines and the subsequent purification of genomic DNA associated with repressive and activating histone modifications for further analysis. This procedure can be widely applied to a variety of histone marks to assess both activating and repressive modifications in the context of myeloid leukaemia.

Related Concepts

Histone H7
Post-Translational Protein Processing
Acute Myeloid Leukemia, M1
Nested Polymerase Chain Reaction
Sequence Determinations, DNA
Chromatin Immunoprecipitation
High-Throughput Nucleotide Sequencing
Enzyme Repression
Histones
Leukemia, Myelocytic, Acute

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