Analysis of HLA-G long-read genomic sequences in mother-offspring pairs with preeclampsia.

Scientific Reports
Ayako NishizawaJunichi Sugawara

Abstract

Preeclampsia is a pregnancy-induced disorder that is characterized by hypertension and is a leading cause of perinatal and maternal-fetal morbidity and mortality. HLA-G is thought to play important roles in maternal-fetal immune tolerance, and the associations between HLA-G gene polymorphisms and the onset of pregnancy-related diseases have been explored extensively. Because contiguous genomic sequencing is difficult, the association between the HLA-G genotype and preeclampsia onset is controversial. In this study, genomic sequences of the HLA-G region (5.2 kb) from 31 pairs of mother-offspring genomic DNA samples (18 pairs from normal pregnancies/births and 13 from preeclampsia births) were obtained by single-molecule real-time sequencing using the PacBio RS II platform. The HLA-G alleles identified in our cohort matched seven known HLA-G alleles, but we also identified two new HLA-G alleles at the fourth-field resolution and compared them with nucleotide sequences from a public database that consisted of coding sequences that cover the 3.1-kb HLA-G gene span. Intriguingly, a potential association between preeclampsia onset and the poly T stretch within the downstream region of the HLA-G*01:01:01:01 allele was found. Our study...Continue Reading

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Citations

Apr 14, 2021·Journal of Leukocyte Biology·Derek MillerNardhy Gomez-Lopez

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Methods Mentioned

BETA
genotyping
PCR
amplicon
amplicon sequencing
electrophoresis

Software Mentioned

Long _ Amplicon _ Analysis in SMRT
Binding Calculator
ClustalW
GnomAD
WebSTR
PacBio RS
RS
SMRT
BLAST
Primer

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