Analysis of human B cell response to recombinant Leishmania LPG3

Asian Pacific Journal of Tropical Medicine
Mostafa Haji FatahalihaMehdi Yousefi

Abstract

To evaluate the capability of recombinant Leishmania LPG3 and its fragments in the activation of B cells. In the present study, human B cells were purified from peripheral blood of 10 adult healthy subjects using magnetic-activated cell sorting technique. Subsequently, purified B cells were treated with recombinant LPG3, and its N-terminal and C-terminal fragments at different concentrations (2, 10 and 20 μg/mL). B cell activation was assessed through expression of CD69 molecule by flow cytometry and secretion of IL-6, TNF-α and IL-10 cytokines via enzyme-linked immunosorbent assay following treatment with recombinant antigens. Our results showed that while the recombinant LPG-3 could significantly increase the production of IL-6 and TNF-α (P < 0.05) in B cells, it had no effect on the secretion of IL-10 by B cells. Our study indicated that recombinant LPG-3 and especially its N-terminal fragment could stimulate B cell response as an important immune response component against leishmaniasis. Thus, it seems that it can be considered as an effective adjuvant in vaccine developments against leishmaniasis.

References

Jan 1, 1992·Clinical and Experimental Immunology·H O Akuffo, S F Britton
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·I VouldoukisM D Mossalayi
Jun 1, 1997·Infection and Immunity·N H MoskowitzS L Reiner
Sep 24, 2002·Nature Immunology·Simon FillatreauStephen M Anderton
Sep 10, 2005·Trends in Parasitology·Simon L CroftJulio A Urbina
Jan 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Atsushi Mizoguchi, Atul K Bhan
Jul 29, 2006·American Journal of Public Health·Irina Serdobova, Marie-Paule Kieny
Mar 14, 2007·International Journal of Antimicrobial Agents·Sonimar NateraAlicia Ponte-Sucre
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jason K WhitmireRafi Ahmed
Dec 8, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Catherine RonetPascal Launois
May 14, 2010·The Journal of Biological Chemistry·Alexander J BankovichJason G Cyster
May 22, 2010·Trends in Parasitology·Alexandre B ReisJavier Moreno
Apr 24, 2012·Journal of Parasitology Research·Luis H FrancoDario S Zamboni
Jun 14, 2012·PloS One·Jorge AlvarUNKNOWN WHO Leishmaniasis Control Team

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Citations

Sep 10, 2016·Immunological Investigations·Abolfazl MiahipourMehdi Yousefi
Sep 28, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Maryam HosseiniMehdi Yousefi

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